Andrey Klymchenko 🇺🇦's banner

Andrey Klymchenko 🇺🇦

@aklymchenko • 3,004 subscribers

CNRS senior researcher @unistra @cnrs. Working on fluorescent probes, organic nanoparticles, biomembranes, biosensing & bioimaging ⚗️🧬🔬 #ERC #BrightSens

Shorts

Since long time we tried to achieve “permanent” labelling of plasma membranes. Now it become possible with MemGraft probes, which exploit a new principle of lipid-directed covalent fluorescent labeling of membrane proteins. The MemGraft probes are compatible with fixation, permeabilization, trypsinization, presence of serum in the medium and enable long-term cell imaging, co-culture of cells and their imaging in multiple colors, cell barcoding and cell manipulation. Congrats and thanks to co-authors: Nathan Aknine Nathan Aknine & Remi Pelletier. - CNRS 🌍 CNRS 🌍_Alsace LBP UMR 7021 Just out in biorXiv:

Since long time we tried to achieve “permanent” labelling of plasma membranes. Now it become possible with MemGraft probes, which exploit a new principle of lipid-directed covalent fluorescent labeling of membrane proteins. The MemGraft probes are compatible with fixation, permeabilization, trypsinization, presence of serum in the medium and enable long-term cell imaging, co-culture of cells and their imaging in multiple colors, cell barcoding and cell manipulation. Congrats and thanks to co-authors: Nathan Aknine Nathan Aknine & Remi Pelletier. - CNRS 🌍 CNRS 🌍_Alsace LBP UMR 7021 Just out in biorXiv:

43,007 views

An Account on our efforts in fluorescent probes for plasma & organelle membranes is out in Acc Chem Res ACS Publications. Big thanks to colleagues of my team & collaborators for years of fruitful work! - CNRS Alsace European Research Council (ERC) ANR - Agence nationale de la recherche

An Account on our efforts in fluorescent probes for plasma & organelle membranes is out in Acc Chem Res ACS Publications. Big thanks to colleagues of my team & collaborators for years of fruitful work! - CNRS Alsace European Research Council (ERC) ANR - Agence nationale de la recherche

32,440 views

I am thrilled that our work on MemGraft probes for “permanent” fluorescent labelling of plasma membranes is out in JACS Au! We established a concept of lipid-directed covalent labeling of plasma membranes. Open access: MemGraft probes allow cell fixation, permeabilization, trypsinization, presence of serum, long-term imaging, co-culture, cell barcoding and magnetic manipulation. Congratulations and thanks to co-authors: Nathan Aknine and Rémi Pelletier. -, CNRS 🌍, CNRS 🌍_Alsace, LBP UMR 7021, ANR - Agence nationale de la recherche

I am thrilled that our work on MemGraft probes for “permanent” fluorescent labelling of plasma membranes is out in JACS Au! We established a concept of lipid-directed covalent labeling of plasma membranes. Open access: MemGraft probes allow cell fixation, permeabilization, trypsinization, presence of serum, long-term imaging, co-culture, cell barcoding and magnetic manipulation. Congratulations and thanks to co-authors: Nathan Aknine and Rémi Pelletier. -, CNRS 🌍, CNRS 🌍_Alsace, LBP UMR 7021, ANR - Agence nationale de la recherche

13,693 views