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A one minute primer on how #AutoSpectral improves spectral flow cytometry unmixing by using per-cell calculation of autofluroscence spectra. Use our R package and get higher quality spectral data, easy as that!

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Adrian Liston

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12,596 görüntüleme • 4 ay önce •via X (Twitter)

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Ari (Ari Wagen) made an interface for IR spectral predictions that's so easy to use that it's hypnotizing. I measured and computed dozens of IR spectra in my PhD, perhaps hundreds, and this is the most intuitive way to interact with the data that I've ever encountered:
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Ari (Ari Wagen) made an interface for IR spectral predictions that's so easy to use that it's hypnotizing. I measured and computed dozens of IR spectra in my PhD, perhaps hundreds, and this is the most intuitive way to interact with the data that I've ever encountered:

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THE SUNRISE IS THE MOST POTENT CIRCADIAN STIMULUS. A 2020 paper published in Current Biology identified a previously unknown retinal circuit explaining precisely why the spectral contrast of sunrise so powerfully entrains the circadian system. The key neuron is the M1 intrinsically photosensitive retinal ganglion cell (ipRGC), which projects directly to the suprachiasmatic nucleus (SCN), the brain’s master circadian pacemaker. In primates, M1 ipRGCs are wired to perform a spectral comparison between yellow/red and blue light via two simultaneous pathways. ***This cone-based circuit is just one of several mechanisms by which morning light entrains the circadian system. Going outside after sunrise still delivers meaningful melanopsin stimulation as overall irradiance builds, and that sustained signal remains highly beneficial. The spectral contrast mechanism described here is simply the most precisely tuned and potent stimulus the system has, one that outdoor morning light delivers far more effectively than any indoor environment. As yellow and red wavelengths increase at sunrise, L and M cones drive LM-ON bipolar cells, which directly excite the M1 ipRGC. Simultaneously, the relative decrease in short-wavelength light reduces S-cone activation, quieting S-ON bipolar cells, which reduces drive to a newly identified S-cone amacrine cell, releasing its inhibitory input onto the ipRGC. Disinhibition and excitation converge on the same cell simultaneously. Melanopsin, the ipRGC’s intrinsic photopigment peaking at ~480nm, is slower and requires sustained high irradiance, taking over as morning light intensity builds. The result is a temporal relay: the cone circuit delivers a rapid spectral signal at the horizon transition, then melanopsin sustains entrainment through the morning. No indoor lighting replicates this. The sunrise delivers the exact stimulus this circuit evolved over hundreds of millions of years to detect. #science #circadian #sunrise #neuroscience #biology

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When the Lorenz Attractor Becomes a Cosmos Many of us have seen plots of the famous Lorenz system that famous butterfly-shaped chaos: ẋ = σ(y - x) ẏ = x(ρ - z) - y ż = xy - βz But here, we went one layer deeper. At every point (x, y, z), we computed the local Jacobian matrix, J(x, y, z) = [ -σ σ 0 ] [ ρ - z -1 - x ] [ y x - β ] Then we found its eigenvalues λ and plotted them in the complex plane. Re(λ) tells us whether nearby motion is locally growing or decaying. Im(λ) tells us whether that local motion is rotating. That gives us the spectral flow of the Lorenz system and not the attractor itself, but the hidden local linear geometry driving its instability. When I saw that flow, it looked strangely cosmic with two mirrored swarms curling like particles trapped in a mathematical whirlpool. So I mapped that spectral flow onto our living fabric, not as Einstein Spacetime this time, but as a creative geometric stage for chaos itself. #LorenzSystem #ChaosTheory #DynamicalSystems #Eigenvalues #SpectralFlow #ComplexPlane #NonlinearDynamics #Mathematics #Physics

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We wanted to share a video of one of the very best features in Atlas, and that's our cross-chain swap! Here we demonstrate how quick and easy it is to swap from Solana to PulseChain! We also give you a sneak preview of our beautiful charting package and our live customer support! Volume on!

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A massive 350,000 square foot data center in Massachusetts is nicknamed “The Dungeon” by residents The data center promised it would provide lots of new jobs to the community. Only about 12 people work there…. The data center is causing constant visible pollution and a lot of noise to residents It’s using about 120,000 gallons of water per day and using as much power as 40,000 American homes Residents were lied to Get this, the data center is buying nearby homes and properties to expand….

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