New work from the #WeinsteinLab is on biorxiv! With MAJOR help from @ZebrafishMK and Aurora Kraus : The axillary lymphoid organ - an external, experimentally accessible immune organ in the zebrafish. Tweetorial below. #Zebrafish

Why is it so cool? Because it is on the OUTSIDE of the fish, and is small and transparent, making it great for imaging. Here is a close look at the blood and lymphatic vessels in the ALO.

We combined single-cell RNAseq, HCR, transgenic lines, TEM, and array tomography (below) to identify the cells that make up the ALO. Here is a close look at the cells in the dermal cortex. A big thanks to collaborators @KedarNarayan2 and Chris Dell at NCI

Interestingly, we noticed that solitary chemosensory cells (like the cells in taste buds) in the ALO have two microvilli extending into the environment! We also captured goblet cells releasing mucus!

Here is a movie showing immune cells crawling along the reticular network in the ALO.

We looked at several different immune cells in the ALO, here are B-cells.

Take a look at our preprint for more cool images and movies. A HUGE thanks to Brant, @ZebrafishMK and @aurora_kraus, as well as all of our AWESOME collaborators and coauthors, some are on Twitter - @vg_biomarina @DNLumbantobing @m_marvel @KedarNarayan2 @TFordRobertson

@ZebrafishMK @aurora_kraus Great work!! Is this orgn present in most fishes?

@ZebrafishMK @aurora_kraus Thanks @MadS100tist ! Flaming Elmo AND an expletive 💯