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Sergiu P. Pasca

@Sergiu_P_Pasca • 14,356 subscribers

Kenneth T. Norris Prof of Psychiatry @Stanford; Uytengsu Director of @BrOrganogenesis; Physician-Scientist; Seeking to understand human brain assembly & disease

Shorts

Today we are sharing our most complex #assembloid to date: a 4-part model of the human ascending neural somatosensory pathway generated by functionally integrating stem cell-derived cerebral cortical, thalamic, spinal and sensory #organoids. Check out the bioRxiv and a short video of activity as these parts self-assemble Work led by the multi-talented Ji-il Kim & Kent Imaizumi

Today we are sharing our most complex #assembloid to date: a 4-part model of the human ascending neural somatosensory pathway generated by functionally integrating stem cell-derived cerebral cortical, thalamic, spinal and sensory #organoids. Check out the bioRxiv and a short video of activity as these parts self-assemble Work led by the multi-talented Ji-il Kim & Kent Imaizumi

39,715 görüntüleme

Cells located in the midline of the embryo play crucial roles in development. These cells often influence cell fate decisions and guide axons to establish bilateral connections. Despite their importance, studying these transient cells and their complex interactions has been challenging. Today, we introduce human midline #assembloids—3D self-organizing cellular clusters derived from stem cells by the functional integration of floor plate #organoids and spinal cord #organoids. We demonstrate that midline assembloids enable cell specification and axon guidance in human neurons. Additionally, we use these assembloids to screen and identify human-enriched factors that control these processes. Work led by the incredible Massimo Onesto & Neal Amin!! More details in the preprint 👇

Cells located in the midline of the embryo play crucial roles in development. These cells often influence cell fate decisions and guide axons to establish bilateral connections. Despite their importance, studying these transient cells and their complex interactions has been challenging. Today, we introduce human midline #assembloids—3D self-organizing cellular clusters derived from stem cells by the functional integration of floor plate #organoids and spinal cord #organoids. We demonstrate that midline assembloids enable cell specification and axon guidance in human neurons. Additionally, we use these assembloids to screen and identify human-enriched factors that control these processes. Work led by the incredible Massimo Onesto & Neal Amin!! More details in the preprint 👇

15,805 görüntüleme

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There are now hundreds of genes associated with autism & neurodevelopmental disorders but what key processes in brain development they each disrupt remains unknown. In an article in nature today, we start tackling this question by combining forebrain #assembloids with CRISPR screens to map >400 disease genes onto stages of human cortical interneuron development. Using engineered lines, regionalized neural #organoids and over 1,000 #assembloids, we discovered a group of disease genes that interfere with GABAergic neuron generation or migration, including some surprising disease mechanisms. Check out the video below for details. This work was led by the fearless Xiangling Meng in the lab!! In collaboration with Bassik lab & Georgia Panagiotakos lab! Article here: Supported by the Stanford Brain Organogenesis Center Wu Tsai Neurosciences Institute Stanford Bio-X New York Stem Cell Foundation Research Institute Biohub

There are now hundreds of genes associated with autism & neurodevelopmental disorders but what key processes in brain development they each disrupt remains unknown. In an article in nature today, we start tackling this question by combining forebrain #assembloids with CRISPR screens to map >400 disease genes onto stages of human cortical interneuron development. Using engineered lines, regionalized neural #organoids and over 1,000 #assembloids, we discovered a group of disease genes that interfere with GABAergic neuron generation or migration, including some surprising disease mechanisms. Check out the video below for details. This work was led by the fearless Xiangling Meng in the lab!! In collaboration with Bassik lab & Georgia Panagiotakos lab! Article here: Supported by the Stanford Brain Organogenesis Center Wu Tsai Neurosciences Institute Stanford Bio-X New York Stem Cell Foundation Research Institute Biohub

Sergiu P. Pasca

165,635 görüntüleme • 2 yıl önce

Today we introduce human neural loop #assembloids created from 4 parts derived from stem cells to study the cortico-striato-midbrain-thalamo-cortical pathway and to model neurodevelopmental disease Work led by the remarkable Ji-Il Kim and Yuki Miura in the lab. Also in collaboration with the Marius Pachitariu lab at HHMI | Janelia Video shows a four-part neural assembloid displaying emergent synchronized activity after a couple of hundred days of integration

Today we introduce human neural loop #assembloids created from 4 parts derived from stem cells to study the cortico-striato-midbrain-thalamo-cortical pathway and to model neurodevelopmental disease Work led by the remarkable Ji-Il Kim and Yuki Miura in the lab. Also in collaboration with the Marius Pachitariu lab at HHMI | Janelia Video shows a four-part neural assembloid displaying emergent synchronized activity after a couple of hundred days of integration

Sergiu P. Pasca

27,394 görüntüleme • 1 yıl önce

Our latest work is out today in Science Magazine: a self-organizing system that models organizer-driven patterning and commissural axon guidance in human neural development. This was achieved with human midline #assembloids by combining floor plate and spinal cord #organoids from pluripotent stem cells. A key advance is the three-part configuration (a floor plate squeezed in between two spinal cords), which enables human axons to cross the midline (and the subsequent synchronization of activity between the two parts seen in the video below). This platform opens the door to many applications; here, we used it to screen and identify human-enriched midline crossing regulators. Work led by Massimo Onesto with Neal Amin

Our latest work is out today in Science Magazine: a self-organizing system that models organizer-driven patterning and commissural axon guidance in human neural development. This was achieved with human midline #assembloids by combining floor plate and spinal cord #organoids from pluripotent stem cells. A key advance is the three-part configuration (a floor plate squeezed in between two spinal cords), which enables human axons to cross the midline (and the subsequent synchronization of activity between the two parts seen in the video below). This platform opens the door to many applications; here, we used it to screen and identify human-enriched midline crossing regulators. Work led by Massimo Onesto with Neal Amin

Sergiu P. Pasca

10,312 görüntüleme • 10 ay önce

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