#assembloids
There are now hundreds of genes associated with autism & neurodevelopmental disorders but what key processes in brain development they each disrupt remains unknown. In an article in nature today, we start tackling this question by combining forebrain #assembloids with CRISPR screens to map >400 disease genes onto stages of human cortical interneuron development. Using engineered lines, regionalized neural #organoids and over 1,000 #assembloids, we discovered a group of disease genes that interfere with GABAergic neuron generation or migration, including some surprising disease mechanisms. Check out the video below for details. This work was led by the fearless Xiangling Meng in the lab!! In collaboration with Bassik lab & Georgia Panagiotakos lab! Article here: Supported by the Stanford Brain Organogenesis Center Wu Tsai Neurosciences Institute Stanford Bio-X New York Stem Cell Foundation Research Institute Biohub
Sergiu P. Pasca165,635 Aufrufe • vor 2 Jahren
Today we introduce human neural loop #assembloids created from 4 parts derived from stem cells to study the cortico-striato-midbrain-thalamo-cortical pathway and to model neurodevelopmental disease Work led by the remarkable Ji-Il Kim and Yuki Miura in the lab. Also in collaboration with the Marius Pachitariu lab at HHMI | Janelia Video shows a four-part neural assembloid displaying emergent synchronized activity after a couple of hundred days of integration
Sergiu P. Pasca27,394 Aufrufe • vor 1 Jahr
Our latest work is out today in Science Magazine: a self-organizing system that models organizer-driven patterning and commissural axon guidance in human neural development. This was achieved with human midline #assembloids by combining floor plate and spinal cord #organoids from pluripotent stem cells. A key advance is the three-part configuration (a floor plate squeezed in between two spinal cords), which enables human axons to cross the midline (and the subsequent synchronization of activity between the two parts seen in the video below). This platform opens the door to many applications; here, we used it to screen and identify human-enriched midline crossing regulators. Work led by Massimo Onesto with Neal Amin
Sergiu P. Pasca10,322 Aufrufe • vor 11 Monaten
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