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Check out what Cryo Lithius can do!
111,288 Aufrufe • vor 3 Jahren •via X (Twitter)
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![Can machine learning enable 100-plex cryo-EM structure determination? (Ellen Zhong, Ep #5) (links in reply) this is a podcast filmed with Ellen Zhong, a computer science professor at Princeton, and one of the giants (if not outright creators) of the field of deep-learning applied to cryo-EM particle images i cannot emphasize how lopsided the ratio of (how important this field is) to (how interesting outside observers consider it) is. it's an area of research that i barely understood until last year. but now that i do grasp it, i increasingly believe that Ellen's work (especially in scaling up cryo-EM) will almost certainly end up being a weight-bearing pillar in the future of bio-ML here, we talk about the possibility of running cryo-EM at extremely high scales, what she did during her recent sabbatical at Generate:Biomedicines, her recent interest in areas beyond cryo-EM (cryo-ET and NMR specifically), and more (and thank you to Rush for sponsoring this! check out their preclinical computational tools at Timestamps: [00:00:00] Introduction [00:02:43] What does it mean to apply ML to cryo-EM? [00:04:28] Ab initio reconstruction and conformational heterogeneity [00:15:41] Can we do multiplex cryo-EM structure determination? [00:22:19] Datasets in cryo-EM [00:26:25] Why isn’t there a foundation model for cryo-EM particle analysis? [00:33:07] How much practical usage is there of these cryo-EM models amongst wet-lab cryo-EM researchers? [00:40:34] Where can things still improve? [00:46:57] Has deep learning done something in cryo-EM that was previously impossible? [00:48:22] Ellen’s experience in the cryo-EM field [00:53:40] Deep learning in cryo-EM outside of structure determination [00:57:32] 3D volume reconstruction versus residue assignment in cryo-EM [01:00:26] What did Ellen do during her sabbatical at Generate Biomedicines? [01:07:07] Ellen’s research in cryo-ET [01:13:54] Ellen’s research in NMR [01:21:05] How did Ellen get into the cryo-EM field? [01:26:57] Why did Ellen go back to graduate school? [01:32:17] What makes Ellen more confident about trusting an external cryo-EM paper?](https://image.24vids.com/tw-1987923818866721138/amplify_video_thumb/1987908501759287296/img/Au3741M4cUZX2m1N.jpg)
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