Video wird geladen...

Video konnte nicht geladen werden

Zur Startseite

🧬Express barcoding: NextGenPCR revolutionized COVID detection - now it's accelerating DNA barcoding🐝🐞! See how 2 students barcode 250 flies 🪰 in 6 hrs with equipment that fits on a lab bench: better and faster than morphospecies sorting. Video 1 of 2:

RudolfMeier15's profile picture

Rudolf Meier @rudolf-meier.bsky.soci

2,350 subscribers

27,347 Aufrufe • vor 3 Jahren •via X (Twitter)

Wissenschaft & Technologie
LIVE

Anya Rossi• Live Now

Private livecam show

6 Kommentare

Profilbild von Caterino Arthropod Biodiversity Lab
Caterino Arthropod Biodiversity Labvor 3 Jahren

Nice, economical protocol. To me the only really questionable step is the HotShot extraction - it doesn't sound like long-term DNA preservation is very good. Any new ideas on that?

Profilbild von Rudolf Meier
Rudolf Meiervor 3 Jahren

This protocol is optimized for speed. If high quality DNA is needed for other purposes, one has to add the time for DNA extraction to the 6 hours.

Profilbild von Fedor Čiampor jr
Fedor Čiampor jrvor 3 Jahren

We learned it from you and it really works like this. Awesome. Thanks 👍

Profilbild von Florian Leese
Florian Leesevor 3 Jahren

@EckartStolle The future is here ☀️ Great work @RudolfMeier15 and team 👍🏼

Profilbild von Nathan K. Lujan
Nathan K. Lujanvor 3 Jahren

Approximate total cost for setup minus typical lab materials?

Profilbild von Rudolf Meier
Rudolf Meiervor 3 Jahren

Cost differs a lot between countries and is best determined for each country (but rough consumable costs are in the paper). The manuscript also disucsses how to trade off cost for time given that faster always means more expensive.

Ähnliche Videos

Express DNA barcoding published! We combine DNA leaching, NextGenPCR, and MinION to go from specimens to barcodes in hours without breaking the bank. Say hello to fast pest identification, food authentication and species sorting in the field. Oxford Nanopore
1:38

Express DNA barcoding published! We combine DNA leaching, NextGenPCR, and MinION to go from specimens to barcodes in hours without breaking the bank. Say hello to fast pest identification, food authentication and species sorting in the field. Oxford Nanopore

Rudolf Meier @rudolf-meier.bsky.soci

24,609 Aufrufe • vor 2 Jahren

ONTbarcoder 2.0: Real-time DNA barcoding with MinION´s R10.4. Now you can find out what species are in your sample, while you are still sequencing. Get more and better barcodes faster than ever.
1:32

ONTbarcoder 2.0: Real-time DNA barcoding with MinION´s R10.4. Now you can find out what species are in your sample, while you are still sequencing. Get more and better barcodes faster than ever.

Rudolf Meier @rudolf-meier.bsky.soci

23,177 Aufrufe • vor 3 Jahren

Out now: Real-time DNA barcoding with MinION! DNA barcoding isn't only for fancy labs - you can now discover global biodiversity in your kitchen while frying an egg (almost)! Reminds us of Dorothy Parker´s "Creativity is a wild mind and a disciplined eye"
0:57

Out now: Real-time DNA barcoding with MinION! DNA barcoding isn't only for fancy labs - you can now discover global biodiversity in your kitchen while frying an egg (almost)! Reminds us of Dorothy Parker´s "Creativity is a wild mind and a disciplined eye"

Rudolf Meier @rudolf-meier.bsky.soci

16,895 Aufrufe • vor 2 Jahren

🧬A new imaging technique developed by the Liu Lab uses a novel DNA barcode system to track hundreds of RNA & protein molecules in single cells within thick biological samples, providing a full picture of how these structures are organized inside tissues.
0:33

🧬A new imaging technique developed by the Liu Lab uses a novel DNA barcode system to track hundreds of RNA & protein molecules in single cells within thick biological samples, providing a full picture of how these structures are organized inside tissues.

HHMI | Janelia

79,474 Aufrufe • vor 1 Jahr

🪰 we are living the dream in a room full of flies 🪰 "flies" by cheekface out now where you listen to finer music 🪰 thanks Jeff Rosenstock for honking on bari sax for us
2:19

🪰 we are living the dream in a room full of flies 🪰 "flies" by cheekface out now where you listen to finer music 🪰 thanks Jeff Rosenstock for honking on bari sax for us

america's local band cheekface

69,955 Aufrufe • vor 1 Jahr

🚨 EntoSieve: New automated tool for fast, accurate size-sorting of bulk insect samples! Perfect for boosting DNA megabarcoding & metabarcoding projects. 🧬🔬 DIY low-cost device, gentle on specimens and yet efficient 🦋🐞read here:
0:57

🚨 EntoSieve: New automated tool for fast, accurate size-sorting of bulk insect samples! Perfect for boosting DNA megabarcoding & metabarcoding projects. 🧬🔬 DIY low-cost device, gentle on specimens and yet efficient 🦋🐞read here:

Rudolf Meier @rudolf-meier.bsky.soci

11,931 Aufrufe • vor 1 Jahr

MASA COMES OFF OF THE BENCH AND BITCH SLAPS LUKE WEAVER WITH A SINGLE THAT DRIVES IN 2 SOX LEAD 2-1 IN THE 7TH
0:30

MASA COMES OFF OF THE BENCH AND BITCH SLAPS LUKE WEAVER WITH A SINGLE THAT DRIVES IN 2 SOX LEAD 2-1 IN THE 7TH

Hogdale

629,487 Aufrufe • vor 9 Monaten

HUMAN DNA FOUND IN AMERICAN HOTDOGS 🧬🌭 Clear Foods analyzed 345 hot dog products, and found human DNA in 2% of the samples.
0:37

HUMAN DNA FOUND IN AMERICAN HOTDOGS 🧬🌭 Clear Foods analyzed 345 hot dog products, and found human DNA in 2% of the samples.

DramaAlert

1,018,930 Aufrufe • vor 1 Jahr

Gymskin was BAFFLED as he discovered that flies only live for 1–2 days 🪰😂 “Why do they hang around lumps of 💩? They’re wasting their lives”
0:20

Gymskin was BAFFLED as he discovered that flies only live for 1–2 days 🪰😂 “Why do they hang around lumps of 💩? They’re wasting their lives”

clipmate

220,030 Aufrufe • vor 2 Monaten

"It's a permanent fixture of their genome now" "Can the little pieces of DNA that are in the [Pfizer mRNA] vaccine ever get into the human genome?": “The Pfizer vaccine is contaminated with Plasmid DNA, it's not just mRNA...it's got bits of DNA in it [and I know] because I sequenced it in my own lab” Phillip Buckhaults, PhD Biochemistry and Molecular Biology. Scientist & Professor of Molecular Biology & Genetics
0:58

"It's a permanent fixture of their genome now" "Can the little pieces of DNA that are in the [Pfizer mRNA] vaccine ever get into the human genome?": “The Pfizer vaccine is contaminated with Plasmid DNA, it's not just mRNA...it's got bits of DNA in it [and I know] because I sequenced it in my own lab” Phillip Buckhaults, PhD Biochemistry and Molecular Biology. Scientist & Professor of Molecular Biology & Genetics

Humanspective

39,623 Aufrufe • vor 11 Monaten

I will not rest until he is in prison.. (NEW) Fauci says it’s a LIE the mRNA COVID Vaccines Alter your DNA 🔴 1.) In Arizona, The GOP "Ban the Jab" resolution was passed calling the Covid Vaccines a Biological Weapon which Alters your DNA ( 🔴 2.) The Surgeon General of Florida sounded the Alarm that the mRNA Covid Vaccines are Altering and Installing Foreign DNA into your Cell’s —— “These vaccines are the ANTI-CHRIST of all products” • The DNA hangs on with the mRNA and is getting delivered into people’s cells with the lipid nano-particles… Therefore BILLIONS of DNA fragments are literally changing your own DNA —— “The more you get it… the more DNA fragments enter into your cells” (Video: 🔴 3.) The Florida Grand Jury report on Covid-19 finds there were excessive amounts of DNA Contamination in various mRNA Covid vaccine batches… DNA Contamination like this “can become incorporated into the genome of the recipient”, hence, it alters your DNA. (Source: 🔴 4.) Pfizer’s own CEO Albert Bourla bragged about the "DNA editing" potential of mRNA vaccine technology • “There’s a third application which is for people that they are born with a mistake in their DNA code.“ (Video: I can go on and on… Fauci Clip
0:39

I will not rest until he is in prison.. (NEW) Fauci says it’s a LIE the mRNA COVID Vaccines Alter your DNA 🔴 1.) In Arizona, The GOP "Ban the Jab" resolution was passed calling the Covid Vaccines a Biological Weapon which Alters your DNA ( 🔴 2.) The Surgeon General of Florida sounded the Alarm that the mRNA Covid Vaccines are Altering and Installing Foreign DNA into your Cell’s —— “These vaccines are the ANTI-CHRIST of all products” • The DNA hangs on with the mRNA and is getting delivered into people’s cells with the lipid nano-particles… Therefore BILLIONS of DNA fragments are literally changing your own DNA —— “The more you get it… the more DNA fragments enter into your cells” (Video: 🔴 3.) The Florida Grand Jury report on Covid-19 finds there were excessive amounts of DNA Contamination in various mRNA Covid vaccine batches… DNA Contamination like this “can become incorporated into the genome of the recipient”, hence, it alters your DNA. (Source: 🔴 4.) Pfizer’s own CEO Albert Bourla bragged about the "DNA editing" potential of mRNA vaccine technology • “There’s a third application which is for people that they are born with a mistake in their DNA code.“ (Video: I can go on and on… Fauci Clip

MJTruthUltra

17,593 Aufrufe • vor 1 Jahr

Funny how Pika text-to-video and image-to-video is arguably better than Runway’s and it’s built by a team of 4 students with $200 million less dollars than Runway has.
1:24

Funny how Pika text-to-video and image-to-video is arguably better than Runway’s and it’s built by a team of 4 students with $200 million less dollars than Runway has.

Nick St. Pierre

238,665 Aufrufe • vor 2 Jahren

Winning runs in our DNA 🧬 Time to lock in and climb higher on Day 2 🚀 #FuelTheiQOOSouL #iQOO #iQOOSouL #MonsterEnergy
0:27

Winning runs in our DNA 🧬 Time to lock in and climb higher on Day 2 🚀 #FuelTheiQOOSouL #iQOO #iQOOSouL #MonsterEnergy

Team iQOOSouL

12,081 Aufrufe • vor 8 Monaten

6, non gimmicky ways to get faster 💨 Come in to see firsthand for yourself how we develop FAST athletes! 1) Get strong. Idc what movements you do, but get strong on 1 and 2 legs. FORCE production is vital. 2) Use accommodating resistance and dynamic effort. 3) Loaded jumps on 1 and 2 legs. POWER. 4) Plyometrics- intensive and extensive plyos on 1 and 2 legs. Rate of force development is vital. 5) Bounds/Skips and any rhythm drill. 6) Most importantly…SPRINT!
0:23

6, non gimmicky ways to get faster 💨 Come in to see firsthand for yourself how we develop FAST athletes! 1) Get strong. Idc what movements you do, but get strong on 1 and 2 legs. FORCE production is vital. 2) Use accommodating resistance and dynamic effort. 3) Loaded jumps on 1 and 2 legs. POWER. 4) Plyometrics- intensive and extensive plyos on 1 and 2 legs. Rate of force development is vital. 5) Bounds/Skips and any rhythm drill. 6) Most importantly…SPRINT!

Joe Aratari

33,696 Aufrufe • vor 1 Jahr

Flies sniff out the 'what' and 'where' of other flies. Now out Lovely new recordings of neuronal responses to a freely-walking male confirm active contrast between two antennae allows flies to 'see' each other using smell. Cell @MRC_LMB 1/2
0:38

Flies sniff out the 'what' and 'where' of other flies. Now out Lovely new recordings of neuronal responses to a freely-walking male confirm active contrast between two antennae allows flies to 'see' each other using smell. Cell @MRC_LMB 1/2

Greg Jefferis

42,986 Aufrufe • vor 3 Jahren

🚀 Now live: Full-Stack + Stripe on MiniMax Agent and more! 1. Full-Stack + Stripe → Build monetizable apps in 1 sentence 2. PPTX Export → Better than top tools 3. Performance ↑ 30% faster, 23% leaner 4. Browser Agent → Now self-hosted, smarter & cheaper Try it now:
0:26

🚀 Now live: Full-Stack + Stripe on MiniMax Agent and more! 1. Full-Stack + Stripe → Build monetizable apps in 1 sentence 2. PPTX Export → Better than top tools 3. Performance ↑ 30% faster, 23% leaner 4. Browser Agent → Now self-hosted, smarter & cheaper Try it now:

MiniMax (official)

787,588 Aufrufe • vor 11 Monaten

🧬🦎EVOLUTIONARY CONVERGENCE🐊🧬 Is it really that hard to believe humanoid Reptilians exist? Some scientists hypothesise that evolution is not random and will eventually evolve based on optimum designs. The humanoid form is an excellent design and is ubiquitous throughout the universe; standing upright with 2 legs, 2 arms, 2 ears, 2 eyes, 1 head, 1 nose, 1 mouth etc. is the norm and seems to be written into the fabric of our reality/matrix. While there are indeed many aliens out there that break the mould and look vastly different than us, Homo sapiens are part of a much larger family and we share common DNA with other humanoid races. The creation of Homo sapiens was a group effort by multiple aliens/ETs (see below) but that doesn’t mean evolutionary convergence isn’t possible over long periods of time. Most Earth scientists are still uninformed regarding this fascinating subject but they will soon learn the truth once we make official contact.🧬🦎👽🐊🧬 “May you live in interesting times.” ~ Ancient Chinese curse
3:50

🧬🦎EVOLUTIONARY CONVERGENCE🐊🧬 Is it really that hard to believe humanoid Reptilians exist? Some scientists hypothesise that evolution is not random and will eventually evolve based on optimum designs. The humanoid form is an excellent design and is ubiquitous throughout the universe; standing upright with 2 legs, 2 arms, 2 ears, 2 eyes, 1 head, 1 nose, 1 mouth etc. is the norm and seems to be written into the fabric of our reality/matrix. While there are indeed many aliens out there that break the mould and look vastly different than us, Homo sapiens are part of a much larger family and we share common DNA with other humanoid races. The creation of Homo sapiens was a group effort by multiple aliens/ETs (see below) but that doesn’t mean evolutionary convergence isn’t possible over long periods of time. Most Earth scientists are still uninformed regarding this fascinating subject but they will soon learn the truth once we make official contact.🧬🦎👽🐊🧬 “May you live in interesting times.” ~ Ancient Chinese curse

Reptile Hybrid

55,800 Aufrufe • vor 1 Jahr

🚨 Anthropic's CEO: "software engineering will be fully automated in 12 months." two types of people right now: type 1: uses Claude Code with basic prompts. scared about the future. type 2: knows all 35 commands, tricks, and workflows. ships faster than a team of 5. type 1 gets replaced in 12 months. type 2 does the replacing. I wrote a full guide on how to start. zero experience needed.
0:58

🚨 Anthropic's CEO: "software engineering will be fully automated in 12 months." two types of people right now: type 1: uses Claude Code with basic prompts. scared about the future. type 2: knows all 35 commands, tricks, and workflows. ships faster than a team of 5. type 1 gets replaced in 12 months. type 2 does the replacing. I wrote a full guide on how to start. zero experience needed.

Khairallah AL-Awady

3,379,965 Aufrufe • vor 2 Monaten