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🧬Express barcoding: NextGenPCR revolutionized COVID detection - now it's accelerating DNA barcoding🐝🐞! See how 2 students barcode 250 flies 🪰 in 6 hrs with equipment that fits on a lab bench: better and faster than morphospecies sorting. Video 1 of 2:

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Rudolf Meier @rudolf-meier.bsky.soci

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27,347 просмотров • 3 лет назад •via X (Twitter)

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Комментарии: 6

Фото профиля Caterino Arthropod Biodiversity Lab
Caterino Arthropod Biodiversity Lab3 лет назад

Nice, economical protocol. To me the only really questionable step is the HotShot extraction - it doesn't sound like long-term DNA preservation is very good. Any new ideas on that?

Фото профиля Rudolf Meier
Rudolf Meier3 лет назад

This protocol is optimized for speed. If high quality DNA is needed for other purposes, one has to add the time for DNA extraction to the 6 hours.

Фото профиля Fedor Čiampor jr
Fedor Čiampor jr3 лет назад

We learned it from you and it really works like this. Awesome. Thanks 👍

Фото профиля Florian Leese
Florian Leese3 лет назад

@EckartStolle The future is here ☀️ Great work @RudolfMeier15 and team 👍🏼

Фото профиля Nathan K. Lujan
Nathan K. Lujan3 лет назад

Approximate total cost for setup minus typical lab materials?

Фото профиля Rudolf Meier
Rudolf Meier3 лет назад

Cost differs a lot between countries and is best determined for each country (but rough consumable costs are in the paper). The manuscript also disucsses how to trade off cost for time given that faster always means more expensive.

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