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🧬Express barcoding: NextGenPCR revolutionized COVID detection - now it's accelerating DNA barcoding🐝🐞! See how 2 students barcode 250 flies 🪰 in 6 hrs with equipment that fits on a lab bench: better and faster than morphospecies sorting. Video 1 of 2:

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Rudolf Meier @rudolf-meier.bsky.soci

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27,347 görüntüleme • 3 yıl önce •via X (Twitter)

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Caterino Arthropod Biodiversity Lab profil fotoğrafı
Caterino Arthropod Biodiversity Lab3 yıl önce

Nice, economical protocol. To me the only really questionable step is the HotShot extraction - it doesn't sound like long-term DNA preservation is very good. Any new ideas on that?

Rudolf Meier profil fotoğrafı
Rudolf Meier3 yıl önce

This protocol is optimized for speed. If high quality DNA is needed for other purposes, one has to add the time for DNA extraction to the 6 hours.

Fedor Čiampor jr profil fotoğrafı
Fedor Čiampor jr3 yıl önce

We learned it from you and it really works like this. Awesome. Thanks 👍

Florian Leese profil fotoğrafı
Florian Leese3 yıl önce

@EckartStolle The future is here ☀️ Great work @RudolfMeier15 and team 👍🏼

Nathan K. Lujan profil fotoğrafı
Nathan K. Lujan3 yıl önce

Approximate total cost for setup minus typical lab materials?

Rudolf Meier profil fotoğrafı
Rudolf Meier3 yıl önce

Cost differs a lot between countries and is best determined for each country (but rough consumable costs are in the paper). The manuscript also disucsses how to trade off cost for time given that faster always means more expensive.

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