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🧬Express barcoding: NextGenPCR revolutionized COVID detection - now it's accelerating DNA barcoding🐝🐞! See how 2 students barcode 250 flies 🪰 in 6 hrs with equipment that fits on a lab bench: better and faster than morphospecies sorting. Video 1 of 2:

Rudolf Meier @rudolf-meier.bsky.soci

2,350 subscribers

27,347 次观看 • 3 年前 •via X (Twitter)

Anya Rossi• Live Now

Private livecam show

6 条评论

Caterino Arthropod Biodiversity Lab 的头像

Caterino Arthropod Biodiversity Lab3 年前

Nice, economical protocol. To me the only really questionable step is the HotShot extraction - it doesn't sound like long-term DNA preservation is very good. Any new ideas on that?

Rudolf Meier 的头像

Rudolf Meier3 年前

This protocol is optimized for speed. If high quality DNA is needed for other purposes, one has to add the time for DNA extraction to the 6 hours.

Fedor Čiampor jr 的头像

Fedor Čiampor jr3 年前

We learned it from you and it really works like this. Awesome. Thanks 👍

Florian Leese 的头像

Florian Leese3 年前

@EckartStolle The future is here ☀️ Great work @RudolfMeier15 and team 👍🏼

Nathan K. Lujan 的头像

Nathan K. Lujan3 年前

Approximate total cost for setup minus typical lab materials?

Rudolf Meier 的头像

Rudolf Meier3 年前

Cost differs a lot between countries and is best determined for each country (but rough consumable costs are in the paper). The manuscript also disucsses how to trade off cost for time given that faster always means more expensive.

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