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Express DNA barcoding published! We combine DNA leaching, NextGenPCR, and MinION to go from specimens to barcodes in hours without breaking the bank. Say hello to fast pest identification, food authentication and species sorting in the field. Oxford Nanopore

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Rudolf Meier @rudolf-meier.bsky.soci

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24,609 görüntüleme • 2 yıl önce •via X (Twitter)

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Rudolf Meier profil fotoğrafı
Rudolf Meier2 yıl önce

@shump_2 @nanopore Sealing the plates was tricky in the initial experiment. That's why the published paper includes an optimized sealing protocol that worked smoothly for us.

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Greg Tucker-Kellogg (gtuckerkellogg.bsky.social)2 yıl önce

@nanopore Congrats!

Saleh Abohadi profil fotoğrafı
Saleh Abohadi2 yıl önce

@nanopore Hallo😅🌹

Carlos🌍🦠🔬🧪🧫🇪🇺🇪🇸🇮🇹🇺🇲🇬🇧 profil fotoğrafı
Carlos🌍🦠🔬🧪🧫🇪🇺🇪🇸🇮🇹🇺🇲🇬🇧2 yıl önce

@BabyBoomerWritr @RudolfMeier15 @nanopore Certainly!

Dani Paulo profil fotoğrafı
Dani Paulo2 yıl önce

@nanopore Awesome! Would this work for bulk samples?

Benzer Videolar

ONTbarcoder 2.0: Real-time DNA barcoding with MinION´s R10.4. Now you can find out what species are in your sample, while you are still sequencing. Get more and better barcodes faster than ever.
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ONTbarcoder 2.0: Real-time DNA barcoding with MinION´s R10.4. Now you can find out what species are in your sample, while you are still sequencing. Get more and better barcodes faster than ever.

Rudolf Meier @rudolf-meier.bsky.soci

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🧬Express barcoding: NextGenPCR revolutionized COVID detection - now it's accelerating DNA barcoding🐝🐞! See how 2 students barcode 250 flies 🪰 in 6 hrs with equipment that fits on a lab bench: better and faster than morphospecies sorting. Video 1 of 2:
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🧬Express barcoding: NextGenPCR revolutionized COVID detection - now it's accelerating DNA barcoding🐝🐞! See how 2 students barcode 250 flies 🪰 in 6 hrs with equipment that fits on a lab bench: better and faster than morphospecies sorting. Video 1 of 2:

Rudolf Meier @rudolf-meier.bsky.soci

27,347 görüntüleme • 3 yıl önce

Out now: Real-time DNA barcoding with MinION! DNA barcoding isn't only for fancy labs - you can now discover global biodiversity in your kitchen while frying an egg (almost)! Reminds us of Dorothy Parker´s "Creativity is a wild mind and a disciplined eye"
0:57

Out now: Real-time DNA barcoding with MinION! DNA barcoding isn't only for fancy labs - you can now discover global biodiversity in your kitchen while frying an egg (almost)! Reminds us of Dorothy Parker´s "Creativity is a wild mind and a disciplined eye"

Rudolf Meier @rudolf-meier.bsky.soci

16,895 görüntüleme • 2 yıl önce

Sequenced DNA from Egyptian mummies published in the science journal Nature revealed that ancient Egyptian nobility were genetically closer to White Bronze Age populations from the Levant and Europeans than to modern Egyptians. Mummy DNA of Ancient Egypt
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Sequenced DNA from Egyptian mummies published in the science journal Nature revealed that ancient Egyptian nobility were genetically closer to White Bronze Age populations from the Levant and Europeans than to modern Egyptians. Mummy DNA of Ancient Egypt

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🚨Professor Roger Zuñiga: Maria's DNA Is Only 33% Human "It is completely different from a Humans. According to DNA studies, Maria has 33% human DNA, and the rest of the DNA does not correspond to any known species." #ufotwitter #uapX #nazcamummies
1:30

🚨Professor Roger Zuñiga: Maria's DNA Is Only 33% Human "It is completely different from a Humans. According to DNA studies, Maria has 33% human DNA, and the rest of the DNA does not correspond to any known species." #ufotwitter #uapX #nazcamummies

Skywatch Signal

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WHOA! DID YOU HEAR? THEY FOUND HUMAN DNA AND RAT DNA IN 22 BIG-NAME FAST FOOD PLACES!
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WHOA! DID YOU HEAR? THEY FOUND HUMAN DNA AND RAT DNA IN 22 BIG-NAME FAST FOOD PLACES!

Jasper Truth 🇺🇸 🇨🇦

85,154 görüntüleme • 1 yıl önce

how did we go from “i’d say hello… and if he’d say hello back, i’d go in for the kiss” to know they’re celebrating his birthday together today 😭
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how did we go from “i’d say hello… and if he’d say hello back, i’d go in for the kiss” to know they’re celebrating his birthday together today 😭

lily saw oasis 🍋 loves pau

39,431 görüntüleme • 9 ay önce

Kearney Disinformation Correction Yesterday, Aidan Kearney attempted to attack the lack of canine DNA detected on John's clothing by tweeting two misleading claims. 1. Canine Swab Collection Kearney Disinformation: Michael Proctor was the source of DNA collected. Factual Information: The swabs were neither from Proctor's body nor collected by him. The swabs were collected by Maureen Hartnett at the MSP crime lab from John's shirt per instructions of Teri Kuhn at UC Davis and were received packaged appropriately. 2. Inhibitors Caused The Lack of Canine DNA Kearney Disinformation: There were inhibitors on John's clothing that masked the presence of canine DNA. Factual Information: UC Davis was asked to perform two tests: a nuclear DNA test which would be used to identify a specific animal and, if the nuclear DNA test failed to yield results, a mitochondrial DNA test called Meat ID to determine presence of DNA from a variety of species. Nuclear DNA is found in the nucleus of the cell and only 2 copies exist whereas mitochondrial DNA is found in the cytoplasm and is significantly more abundant with hundreds of thousands of copies. Nuclear DNA tests are far more accurate in determining the exact source, ie one dog from another dog, whereas mitochondrial DNA is less specific but will be able to determine if DNA from a specific animal species is present. In this case, the presence of DNA from 12 different animal species, including canine and pig, was tested. The results of the QPCR testing for nuclear DNA were that the swabs "did not indicate the presence of canine (nuclear) DNA, but did indicate the presence of inhibitors. This result is obtained when 1. Canine DNA is degraded, 2. The DNA is obscured by inhibitors, or 3. There is no nuclear DNA present." The less specific test using mitochondrial DNA, Meat ID, was then completed. This test showed no canine DNA and further, showed no inhibitors. In other words, had canine DNA been present, it would have been identified on the Meat ID mitochondrial testing since there were no inhibitors. The below video contains questioning from both the defense and prosecution supporting the identified factual information.
2:02

Kearney Disinformation Correction Yesterday, Aidan Kearney attempted to attack the lack of canine DNA detected on John's clothing by tweeting two misleading claims. 1. Canine Swab Collection Kearney Disinformation: Michael Proctor was the source of DNA collected. Factual Information: The swabs were neither from Proctor's body nor collected by him. The swabs were collected by Maureen Hartnett at the MSP crime lab from John's shirt per instructions of Teri Kuhn at UC Davis and were received packaged appropriately. 2. Inhibitors Caused The Lack of Canine DNA Kearney Disinformation: There were inhibitors on John's clothing that masked the presence of canine DNA. Factual Information: UC Davis was asked to perform two tests: a nuclear DNA test which would be used to identify a specific animal and, if the nuclear DNA test failed to yield results, a mitochondrial DNA test called Meat ID to determine presence of DNA from a variety of species. Nuclear DNA is found in the nucleus of the cell and only 2 copies exist whereas mitochondrial DNA is found in the cytoplasm and is significantly more abundant with hundreds of thousands of copies. Nuclear DNA tests are far more accurate in determining the exact source, ie one dog from another dog, whereas mitochondrial DNA is less specific but will be able to determine if DNA from a specific animal species is present. In this case, the presence of DNA from 12 different animal species, including canine and pig, was tested. The results of the QPCR testing for nuclear DNA were that the swabs "did not indicate the presence of canine (nuclear) DNA, but did indicate the presence of inhibitors. This result is obtained when 1. Canine DNA is degraded, 2. The DNA is obscured by inhibitors, or 3. There is no nuclear DNA present." The less specific test using mitochondrial DNA, Meat ID, was then completed. This test showed no canine DNA and further, showed no inhibitors. In other words, had canine DNA been present, it would have been identified on the Meat ID mitochondrial testing since there were no inhibitors. The below video contains questioning from both the defense and prosecution supporting the identified factual information.

Julie Carpenter

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“Our clients become partners, and we have energy in our DNA.” ENGIE Impact focuses on a holistic approach from procurement to implementing to help clients excel in the energy transition.
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“Our clients become partners, and we have energy in our DNA.” ENGIE Impact focuses on a holistic approach from procurement to implementing to help clients excel in the energy transition.

Climate Group

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Introducing the MinION Mk1D. #nanoporeconf
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Introducing the MinION Mk1D. #nanoporeconf

Oxford Nanopore

24,168 görüntüleme • 2 yıl önce

⚠️ What’s really in your food supply? Fast food across America has tested positive for rat feces, salmonella and human DNA.
2:52

⚠️ What’s really in your food supply? Fast food across America has tested positive for rat feces, salmonella and human DNA.

The Maverick Approach

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CIGARETTE DNA CRACKS 44-YEAR-OLD MURDER CASE In 1982, 13-year-old Sarah Geer was raped and strangled in California. Investigators collected evidence, but DNA profiling was not yet used in court. So the case waited. In 2003, a usable DNA profile was developed. No match in the FBI database. Back on the shelf. Then came genetic genealogy. Investigators compared the crime scene DNA to public family-history databases. Not looking for the suspect. Looking for relatives. They built a family tree, narrowed it to four brothers, and later matched DNA from a discarded cigarette. That DNA matched the crime-scene profile. So police arrested the suspect. The jury deliberated for two hours. Guilty. After 44 years, the technology finally caught up.
4:40

CIGARETTE DNA CRACKS 44-YEAR-OLD MURDER CASE In 1982, 13-year-old Sarah Geer was raped and strangled in California. Investigators collected evidence, but DNA profiling was not yet used in court. So the case waited. In 2003, a usable DNA profile was developed. No match in the FBI database. Back on the shelf. Then came genetic genealogy. Investigators compared the crime scene DNA to public family-history databases. Not looking for the suspect. Looking for relatives. They built a family tree, narrowed it to four brothers, and later matched DNA from a discarded cigarette. That DNA matched the crime-scene profile. So police arrested the suspect. The jury deliberated for two hours. Guilty. After 44 years, the technology finally caught up.

NewsForce

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🧬 Why does DNA remain in vials of COVID-19 mRNA Shots—and why do regulators continue to miss finding and reporting DNA contamination? IMA Senior Fellow Dr. Jessica Rose and genomics researcher Kevin McKernan discuss key findings from their recent paper, published in the Journal of Independent Medicine. Watch now. 'How Did Regulators Miss DNA Contamination in mRNA Vaccines? New Study Explains' - Learn more: RNA:DNA Hybrids Survive Digestion in mRNA Vaccine Manufacturing
25:54

🧬 Why does DNA remain in vials of COVID-19 mRNA Shots—and why do regulators continue to miss finding and reporting DNA contamination? IMA Senior Fellow Dr. Jessica Rose and genomics researcher Kevin McKernan discuss key findings from their recent paper, published in the Journal of Independent Medicine. Watch now. 'How Did Regulators Miss DNA Contamination in mRNA Vaccines? New Study Explains' - Learn more: RNA:DNA Hybrids Survive Digestion in mRNA Vaccine Manufacturing

Dr Dave Cartland BMedSc MBChB Ex-MRCGP

11,649 görüntüleme • 5 ay önce

🚨🚨 EPIC Paul Offit takedown! PLEASE LIKE AND SHARE WIDELY! A point by point analysis of the false claims and lies he presented as scientific fact in his infamous 2024 "DNA fragments in COVID vaccines are completely harmless" video: LIE #1: The mRNA vaccines contain fragments of DNA, but these are in very small quantities, measured in nanograms - Counterargument: Even though the DNA fragments may be in small quantities, their presence still poses a risk. DNA integration into the genome, even in small quantities, could still have long-term, unpredictable effects that we are not yet fully aware of. LIE #2: Impossibility of DNA entering human DNA: For these DNA fragments to integrate into our DNA, they would have to overcome several barriers, including crossing the cytoplasm and nuclear membrane, which is virtually impossible - Counterargument: The entire purpose of the LNP (lipid nanoparticle) in mRNA vaccines is to allow for the mRNA to enter the cytoplasm in order to transcribe the desired proteins. While fragments integrating into human DNA might be low, it's not "impossible." Scientific literature on gene editing technologies, like CRISPR, demonstrates that foreign DNA can be integrated into the genome under certain conditions. LIE #3: Role of innate immune system in rejecting foreign DNA: The innate immune system and enzymes in the cytoplasm work to destroy foreign DNA - Counterargument: While our immune system does recognize and destroy some foreign DNA, the immune response isn't perfect, especially if the foreign DNA is introduced in a manner that is not immediately recognized such as via the LNP. In certain environments or under specific conditions, foreign DNA can evade immune detection. LIE #4: Lack of integrases in mRNA vaccine DNA: The DNA fragments in the mRNA vaccine do not have the necessary integrases to integrate into our genome - Counterargument: While the fragments might lack integrases, it's possible that they could still interact with the genome in other ways, for example, through epigenetic changes or by influencing gene expression as is commonly achieved in laboratory settings. LIE #5: Comparison to naturally occurring foreign DNA: We are constantly exposed to foreign DNA through our food from bacteria, plants, and animals, which is much larger and more abundant than the DNA in vaccines - Counterargument: Although we are exposed to foreign DNA regularly through the digestive tract, the introduction of DNA through vaccination is a more controlled and concentrated process. The body is not naturally prepared to deal with such DNA in the same way it handles the ingestion of plant or animal DNA. LIE #6: Fear-mongering accusation: The physician scientist who raised these concerns was just scaring people unnecessarily - Counterargument: Raising concerns about potential risks is an important part of scientific discourse, especially when it comes to public health. It's better to err on the side of caution and investigate these possibilities rather than dismissing concerns outright, especially when the long-term effects of mRNA vaccines are still being studied. LIE #7: Assurance that these risks are negligible: The DNA fragments from the vaccines are clinically harmless - Counterargument: The assertion that these fragments are harmless is premature. Without long-term studies specifically addressing the potential risks of vaccine-derived DNA integration, it's impossible to claim with certainty that there will be no adverse effects in the future. Paul Offit knows all of this, so WHY IS HE LYING TO THE AMERICAN PUBLIC? Please share this important video with everyone you know and DEMAND TRANSPARENT RESEARCH INTO THE QUESTION OF DNA CONTAMINATION AND INTEGRATION IN THE #COVID #vaccinated POPULATION! @P_J_Buckhaults Kevin McKernan Danny Jones Bret Weinstein Vejon Health - Dr Philip McMillan John Campbell Robert F. Kennedy Jr Steve Kirsch #AwfulOffit
12:57

🚨🚨 EPIC Paul Offit takedown! PLEASE LIKE AND SHARE WIDELY! A point by point analysis of the false claims and lies he presented as scientific fact in his infamous 2024 "DNA fragments in COVID vaccines are completely harmless" video: LIE #1: The mRNA vaccines contain fragments of DNA, but these are in very small quantities, measured in nanograms - Counterargument: Even though the DNA fragments may be in small quantities, their presence still poses a risk. DNA integration into the genome, even in small quantities, could still have long-term, unpredictable effects that we are not yet fully aware of. LIE #2: Impossibility of DNA entering human DNA: For these DNA fragments to integrate into our DNA, they would have to overcome several barriers, including crossing the cytoplasm and nuclear membrane, which is virtually impossible - Counterargument: The entire purpose of the LNP (lipid nanoparticle) in mRNA vaccines is to allow for the mRNA to enter the cytoplasm in order to transcribe the desired proteins. While fragments integrating into human DNA might be low, it's not "impossible." Scientific literature on gene editing technologies, like CRISPR, demonstrates that foreign DNA can be integrated into the genome under certain conditions. LIE #3: Role of innate immune system in rejecting foreign DNA: The innate immune system and enzymes in the cytoplasm work to destroy foreign DNA - Counterargument: While our immune system does recognize and destroy some foreign DNA, the immune response isn't perfect, especially if the foreign DNA is introduced in a manner that is not immediately recognized such as via the LNP. In certain environments or under specific conditions, foreign DNA can evade immune detection. LIE #4: Lack of integrases in mRNA vaccine DNA: The DNA fragments in the mRNA vaccine do not have the necessary integrases to integrate into our genome - Counterargument: While the fragments might lack integrases, it's possible that they could still interact with the genome in other ways, for example, through epigenetic changes or by influencing gene expression as is commonly achieved in laboratory settings. LIE #5: Comparison to naturally occurring foreign DNA: We are constantly exposed to foreign DNA through our food from bacteria, plants, and animals, which is much larger and more abundant than the DNA in vaccines - Counterargument: Although we are exposed to foreign DNA regularly through the digestive tract, the introduction of DNA through vaccination is a more controlled and concentrated process. The body is not naturally prepared to deal with such DNA in the same way it handles the ingestion of plant or animal DNA. LIE #6: Fear-mongering accusation: The physician scientist who raised these concerns was just scaring people unnecessarily - Counterargument: Raising concerns about potential risks is an important part of scientific discourse, especially when it comes to public health. It's better to err on the side of caution and investigate these possibilities rather than dismissing concerns outright, especially when the long-term effects of mRNA vaccines are still being studied. LIE #7: Assurance that these risks are negligible: The DNA fragments from the vaccines are clinically harmless - Counterargument: The assertion that these fragments are harmless is premature. Without long-term studies specifically addressing the potential risks of vaccine-derived DNA integration, it's impossible to claim with certainty that there will be no adverse effects in the future. Paul Offit knows all of this, so WHY IS HE LYING TO THE AMERICAN PUBLIC? Please share this important video with everyone you know and DEMAND TRANSPARENT RESEARCH INTO THE QUESTION OF DNA CONTAMINATION AND INTEGRATION IN THE #COVID #vaccinated POPULATION! @P_J_Buckhaults Kevin McKernan Danny Jones Bret Weinstein Vejon Health - Dr Philip McMillan John Campbell Robert F. Kennedy Jr Steve Kirsch #AwfulOffit

Vaccine Safety Research Foundation

338,841 görüntüleme • 1 yıl önce

🚨 EntoSieve: New automated tool for fast, accurate size-sorting of bulk insect samples! Perfect for boosting DNA megabarcoding & metabarcoding projects. 🧬🔬 DIY low-cost device, gentle on specimens and yet efficient 🦋🐞read here:
0:57

🚨 EntoSieve: New automated tool for fast, accurate size-sorting of bulk insect samples! Perfect for boosting DNA megabarcoding & metabarcoding projects. 🧬🔬 DIY low-cost device, gentle on specimens and yet efficient 🦋🐞read here:

Rudolf Meier @rudolf-meier.bsky.soci

11,931 görüntüleme • 1 yıl önce

🧬 Why does DNA remain in vials of COVID-19 mRNA Shots—and why do regulators continue to miss finding and reporting DNA contamination? IMA Senior Fellow Dr. Jessica Rose (Jessica Rose 🤙) and genomics researcher Kevin McKernan discuss key findings from their recent paper, published in the Journal of Independent Medicine (Journal of Independent Medicine). Watch now.
25:54

🧬 Why does DNA remain in vials of COVID-19 mRNA Shots—and why do regulators continue to miss finding and reporting DNA contamination? IMA Senior Fellow Dr. Jessica Rose (Jessica Rose 🤙) and genomics researcher Kevin McKernan discuss key findings from their recent paper, published in the Journal of Independent Medicine (Journal of Independent Medicine). Watch now.

Independent Medical Alliance

16,339 görüntüleme • 5 ay önce

The Mexican-American War was about slavery, as U.S. white slaveholders migrated to Mexican Texas to preserve the institution of slavery. Racism is in the DNA of America, and racism is in the DNA of white people.
1:18

The Mexican-American War was about slavery, as U.S. white slaveholders migrated to Mexican Texas to preserve the institution of slavery. Racism is in the DNA of America, and racism is in the DNA of white people.

kevin blue

35,179 görüntüleme • 6 ay önce

Prince William and I extracted the DNA from a strawberry. I went to NatureMetrics today to find out how they’re extracting environmental DNA from soil, rivers and the air to learn more about the world and help protect nature 🪴 #science #experiment #chemistry
0:48

Prince William and I extracted the DNA from a strawberry. I went to NatureMetrics today to find out how they’re extracting environmental DNA from soil, rivers and the air to learn more about the world and help protect nature 🪴 #science #experiment #chemistry

Big Manny

4,736,902 görüntüleme • 1 yıl önce

2%human and 1%rodent DNA in McDonalds food
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2%human and 1%rodent DNA in McDonalds food

karma

13,268 görüntüleme • 9 gün önce

UTAH INVESTIGATOR: DNA was collected from a screwdriver and rifle in the Charlie Kirk murder and it was matched to Tyler Robinson
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UTAH INVESTIGATOR: DNA was collected from a screwdriver and rifle in the Charlie Kirk murder and it was matched to Tyler Robinson

The Post Millennial

77,301 görüntüleme • 4 ay önce