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Express DNA barcoding published! We combine DNA leaching, NextGenPCR, and MinION to go from specimens to barcodes in hours without breaking the bank. Say hello to fast pest identification, food authentication and species sorting in the field. Oxford Nanopore

Rudolf Meier @rudolf-meier.bsky.soci

2,352 subscribers

24,609 次观看 • 2 年前 •via X (Twitter)

Anya Rossi• Live Now

Private livecam show

5 条评论

Rudolf Meier 的头像

Rudolf Meier2 年前

@shump_2 @nanopore Sealing the plates was tricky in the initial experiment. That's why the published paper includes an optimized sealing protocol that worked smoothly for us.

Greg Tucker-Kellogg (gtuckerkellogg.bsky.social) 的头像

Greg Tucker-Kellogg (gtuckerkellogg.bsky.social)2 年前

@nanopore Congrats!

Saleh Abohadi 的头像

Saleh Abohadi2 年前

@nanopore Hallo😅🌹

Carlos🌍🦠🔬🧪🧫🇪🇺🇪🇸🇮🇹🇺🇲🇬🇧 的头像

Carlos🌍🦠🔬🧪🧫🇪🇺🇪🇸🇮🇹🇺🇲🇬🇧2 年前

@BabyBoomerWritr @RudolfMeier15 @nanopore Certainly!

Dani Paulo 的头像

Dani Paulo2 年前

@nanopore Awesome! Would this work for bulk samples?

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Kearney Disinformation Correction Yesterday, Aidan Kearney attempted to attack the lack of canine DNA detected on John's clothing by tweeting two misleading claims. 1. Canine Swab Collection Kearney Disinformation: Michael Proctor was the source of DNA collected. Factual Information: The swabs were neither from Proctor's body nor collected by him. The swabs were collected by Maureen Hartnett at the MSP crime lab from John's shirt per instructions of Teri Kuhn at UC Davis and were received packaged appropriately. 2. Inhibitors Caused The Lack of Canine DNA Kearney Disinformation: There were inhibitors on John's clothing that masked the presence of canine DNA. Factual Information: UC Davis was asked to perform two tests: a nuclear DNA test which would be used to identify a specific animal and, if the nuclear DNA test failed to yield results, a mitochondrial DNA test called Meat ID to determine presence of DNA from a variety of species. Nuclear DNA is found in the nucleus of the cell and only 2 copies exist whereas mitochondrial DNA is found in the cytoplasm and is significantly more abundant with hundreds of thousands of copies. Nuclear DNA tests are far more accurate in determining the exact source, ie one dog from another dog, whereas mitochondrial DNA is less specific but will be able to determine if DNA from a specific animal species is present. In this case, the presence of DNA from 12 different animal species, including canine and pig, was tested. The results of the QPCR testing for nuclear DNA were that the swabs "did not indicate the presence of canine (nuclear) DNA, but did indicate the presence of inhibitors. This result is obtained when 1. Canine DNA is degraded, 2. The DNA is obscured by inhibitors, or 3. There is no nuclear DNA present." The less specific test using mitochondrial DNA, Meat ID, was then completed. This test showed no canine DNA and further, showed no inhibitors. In other words, had canine DNA been present, it would have been identified on the Meat ID mitochondrial testing since there were no inhibitors. The below video contains questioning from both the defense and prosecution supporting the identified factual information.

Kearney Disinformation Correction Yesterday, Aidan Kearney attempted to attack the lack of canine DNA detected on John's clothing by tweeting two misleading claims. 1. Canine Swab Collection Kearney Disinformation: Michael Proctor was the source of DNA collected. Factual Information: The swabs were neither from Proctor's body nor collected by him. The swabs were collected by Maureen Hartnett at the MSP crime lab from John's shirt per instructions of Teri Kuhn at UC Davis and were received packaged appropriately. 2. Inhibitors Caused The Lack of Canine DNA Kearney Disinformation: There were inhibitors on John's clothing that masked the presence of canine DNA. Factual Information: UC Davis was asked to perform two tests: a nuclear DNA test which would be used to identify a specific animal and, if the nuclear DNA test failed to yield results, a mitochondrial DNA test called Meat ID to determine presence of DNA from a variety of species. Nuclear DNA is found in the nucleus of the cell and only 2 copies exist whereas mitochondrial DNA is found in the cytoplasm and is significantly more abundant with hundreds of thousands of copies. Nuclear DNA tests are far more accurate in determining the exact source, ie one dog from another dog, whereas mitochondrial DNA is less specific but will be able to determine if DNA from a specific animal species is present. In this case, the presence of DNA from 12 different animal species, including canine and pig, was tested. The results of the QPCR testing for nuclear DNA were that the swabs "did not indicate the presence of canine (nuclear) DNA, but did indicate the presence of inhibitors. This result is obtained when 1. Canine DNA is degraded, 2. The DNA is obscured by inhibitors, or 3. There is no nuclear DNA present." The less specific test using mitochondrial DNA, Meat ID, was then completed. This test showed no canine DNA and further, showed no inhibitors. In other words, had canine DNA been present, it would have been identified on the Meat ID mitochondrial testing since there were no inhibitors. The below video contains questioning from both the defense and prosecution supporting the identified factual information.

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338,869 次观看 • 1 年前

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🧬 Why does DNA remain in vials of COVID-19 mRNA Shots—and why do regulators continue to miss finding and reporting DNA contamination? IMA Senior Fellow Dr. Jessica Rose (Jessica Rose 🤙) and genomics researcher Kevin McKernan discuss key findings from their recent paper, published in the Journal of Independent Medicine (Journal of Independent Medicine). Watch now.

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16,339 次观看 • 5 个月前

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UTAH INVESTIGATOR: DNA was collected from a screwdriver and rifle in the Charlie Kirk murder and it was matched to Tyler Robinson

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77,301 次观看 • 5 个月前

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