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In 2000, a physicist named Michael Elowitz published one of the first synthetic gene circuits, called the "repressilator." By stitching three genes together, he endowed living cells with an artificial rhythm, coaxing them to flash green. Learn how he built it, and even play around with its parameters, in... show more

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"Rahul Ghandy was not trying to defame the Army. He was reading an excerpt from the book of Army Chief"- Priyanka Vadra चल झूठी! The book isn't even published. He was reading an article published in a propaganda tabloid CLAIMING it to be from book & he was doing it deliberately.

"Rahul Ghandy was not trying to defame the Army. He was reading an excerpt from the book of Army Chief"- Priyanka Vadra चल झूठी! The book isn't even published. He was reading an article published in a propaganda tabloid CLAIMING it to be from book & he was doing it deliberately.

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🔥🚨DEVELOPING: Netflix aired footage of a man they claim is a former Michael Jackson ‘associate’ named Vincent Amen who worked at Neverland Ranch from 2002 to 2003 and alleged that Michael gave child ab*se material to a 5-year-old Cascio who later became his personal assistant. In the new “The Verdict” documentary Vincent claims he was put in charge of taking care of the Arvizo family during their stay at the property following media backlash from Gavin Arvizo’s appearance in “Living With Michael Jackson.” Vincent says he was given a bag to dispose and inside he found a nudist magazine called “Naturally.” and after flipping to a video ordering section with titles circled in black marker, included videos called “Nudist Youth Weekend” and “Euro-Nudist Family.” Vincent: “I confronted Frank, I said, ‘Frank, what is this magazine? Because, you know, there’s circles around videos with naked children,’” Amen recounted. “He said, ‘That’s just a phase that Michael and I went through. He circled the videos that he wanted, I ordered them, and it was a phase that we went through.’ They watched them together.”

🔥🚨DEVELOPING: Netflix aired footage of a man they claim is a former Michael Jackson ‘associate’ named Vincent Amen who worked at Neverland Ranch from 2002 to 2003 and alleged that Michael gave child ab*se material to a 5-year-old Cascio who later became his personal assistant. In the new “The Verdict” documentary Vincent claims he was put in charge of taking care of the Arvizo family during their stay at the property following media backlash from Gavin Arvizo’s appearance in “Living With Michael Jackson.” Vincent says he was given a bag to dispose and inside he found a nudist magazine called “Naturally.” and after flipping to a video ordering section with titles circled in black marker, included videos called “Nudist Youth Weekend” and “Euro-Nudist Family.” Vincent: “I confronted Frank, I said, ‘Frank, what is this magazine? Because, you know, there’s circles around videos with naked children,’” Amen recounted. “He said, ‘That’s just a phase that Michael and I went through. He circled the videos that he wanted, I ordered them, and it was a phase that we went through.’ They watched them together.”

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Observation 1: A look at Marquis Fennell, who is a do-it-all type of player. You can see a trick play and him making an accurate throw in the first clip. He can play in the slow, boundary receiver, or running back. He is one of the best ATHs in the state.

Observation 1: A look at Marquis Fennell, who is a do-it-all type of player. You can see a trick play and him making an accurate throw in the first clip. He can play in the slow, boundary receiver, or running back. He is one of the best ATHs in the state.

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One of the key reasons Gene Wilder introduced the limp to Willy Wonka's character in the 1971 hit film "Charlie and the Chocolate Factory" was to make it difficult for the audience to tell if he was lying or not. The limp was a deliberate part of Wilder's performance strategy to keep the audience guessing about Wonka's true intentions and emotions. Wilder wanted to convey a sense of unpredictability and ambiguity in the character. By using the limp as a physical quirk, he could emphasize the idea that Willy Wonka was a complex and enigmatic individual. This made it challenging for the audience to discern whether he was being sincere or putting on an act, which added an extra layer of intrigue to the character's interactions with the children and their families. In essence, the limp served both to create a multifaceted portrayal of Willy Wonka and to contribute to the overall mystique and uncertainty surrounding the character, making Gene Wilder's performance as Wonka all the more captivating and memorable.

One of the key reasons Gene Wilder introduced the limp to Willy Wonka's character in the 1971 hit film "Charlie and the Chocolate Factory" was to make it difficult for the audience to tell if he was lying or not. The limp was a deliberate part of Wilder's performance strategy to keep the audience guessing about Wonka's true intentions and emotions. Wilder wanted to convey a sense of unpredictability and ambiguity in the character. By using the limp as a physical quirk, he could emphasize the idea that Willy Wonka was a complex and enigmatic individual. This made it challenging for the audience to discern whether he was being sincere or putting on an act, which added an extra layer of intrigue to the character's interactions with the children and their families. In essence, the limp served both to create a multifaceted portrayal of Willy Wonka and to contribute to the overall mystique and uncertainty surrounding the character, making Gene Wilder's performance as Wonka all the more captivating and memorable.

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A tourism article from Welcome to Yorkshire and Parkdean Resorts (published 30 June 2026) reveals that the Princess of Wales’s completion of the Three Peaks Challenge has created a massive surge in interest. Google searches for “Three Peaks Challenge” skyrocketed by 942% in just one week. Source: #PrincessCatherine

A tourism article from Welcome to Yorkshire and Parkdean Resorts (published 30 June 2026) reveals that the Princess of Wales’s completion of the Three Peaks Challenge has created a massive surge in interest. Google searches for “Three Peaks Challenge” skyrocketed by 942% in just one week. Source: #PrincessCatherine

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It still remains a mystery to me how Monet did this. 1874. Paris. Monet and a bunch of broke young artists couldn't get into the official Salon because the jury kept rejecting them. So they decided to do their own show. Renoir, Pissarro, Degas, Cézanne - 30 of them renting a photographer named Nadar's studio. Monet puts up this small, sketchy painting from Le Havre. A sunrise, loose brushstrokes, barely finished. He couldn't even think of a proper title, so in the catalog he wrote 'Impression, Sunrise'. A critic named Louis Leroy comes in. He hated it. He writes a sarcastic review in Le Charivari called 'The Exhibition of the Impressionists' and says: "Impression — I was certain of it. I was just telling myself that, since I was impressed, there had to be some impression in it... and what freedom, what ease of workmanship! Wallpaper in its embryonic state is more finished than that seascape." He meant it as an insult. But the insult stuck. And became the name of the biggest art movement of the century.

It still remains a mystery to me how Monet did this. 1874. Paris. Monet and a bunch of broke young artists couldn't get into the official Salon because the jury kept rejecting them. So they decided to do their own show. Renoir, Pissarro, Degas, Cézanne - 30 of them renting a photographer named Nadar's studio. Monet puts up this small, sketchy painting from Le Havre. A sunrise, loose brushstrokes, barely finished. He couldn't even think of a proper title, so in the catalog he wrote 'Impression, Sunrise'. A critic named Louis Leroy comes in. He hated it. He writes a sarcastic review in Le Charivari called 'The Exhibition of the Impressionists' and says: "Impression — I was certain of it. I was just telling myself that, since I was impressed, there had to be some impression in it... and what freedom, what ease of workmanship! Wallpaper in its embryonic state is more finished than that seascape." He meant it as an insult. But the insult stuck. And became the name of the biggest art movement of the century.

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July 16, 1945. At 5:29 in the morning, in a stretch of New Mexico desert so brutal the Spanish had named it the Journey of the Dead Man, the United States detonated the first nuclear weapon in history. They called the test Trinity and they called the bomb itself the Gadget. Nobody was totally sure what would happen. Scientists had a betting pool going on the yield, and a couple of them had quietly worried whether the thing might ignite the atmosphere itself. They went ahead anyway. The flash lit up the mountains brighter than the sun. People felt it 100 miles away, windows rattled in distant towns, and the heat fused the desert sand into a weird green glass that scientists later named trinitite. The Army put out a cover story that an ammunition dump had exploded. Robert Oppenheimer later said that watching it, he thought of a line from Hindu scripture. Now I am become Death, the destroyer of worlds. Another physicist there put it more bluntly and said now we are all sons of bitches. Three weeks later Hiroshima was gone. The world changed at dawn in a desert on this day.

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The journey of creating Game Worlds began 28 years ago. A bunch of people came together under one roof to share their stories with the world. The first games were burned onto CDs and passed from one person to another, bringing together folks with different life stories and backgrounds. Friends gathered around old screens, immersing themselves in something completely unique and new. With enthusiasm and fresh ideas, the Game Worlds expanded. The stories left imprints on people's hearts and souls, inspiring the development team to create even more of them. We've always been in this together — cossacks, heroes, stalkers, and the GSC Game World team. It's you who breathe life into our worlds, and you're the ones who can live our stories and share them with others. We're thrilled to create for you. Yep, you are like the blood cells of our A-Life Game Worlds. So important that without you, nothing would exist. Thanks for being with us on our 28th Anniversary.

The journey of creating Game Worlds began 28 years ago. A bunch of people came together under one roof to share their stories with the world. The first games were burned onto CDs and passed from one person to another, bringing together folks with different life stories and backgrounds. Friends gathered around old screens, immersing themselves in something completely unique and new. With enthusiasm and fresh ideas, the Game Worlds expanded. The stories left imprints on people's hearts and souls, inspiring the development team to create even more of them. We've always been in this together — cossacks, heroes, stalkers, and the GSC Game World team. It's you who breathe life into our worlds, and you're the ones who can live our stories and share them with others. We're thrilled to create for you. Yep, you are like the blood cells of our A-Life Game Worlds. So important that without you, nothing would exist. Thanks for being with us on our 28th Anniversary.

GSC Game World

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A 20-year-old student from China, Li Hao, built an AI speed radar with Claude alone and sold it to a city district for $317,000 He wrote the whole thing in 9 days, spending about $20 on Claude API calls He set an old camera on his balcony, pointed it at the intersection below, and let Claude watch the road Claude tags every car, motorbike and pedestrian in real time, 653 in five minutes, and flags anyone over the limit The moment a car speeds, Claude clips the video, reads the license plate, matches the owner, and emails the fine on its own A normal radar takes one photo and misses half the time. Claude records full video, so there is nothing to dispute, and the fines go out with no operator He walked into the district office with a flash drive and asked for 10 minutes. he left with a contract Every Claude config he used is in the article

A 20-year-old student from China, Li Hao, built an AI speed radar with Claude alone and sold it to a city district for $317,000 He wrote the whole thing in 9 days, spending about $20 on Claude API calls He set an old camera on his balcony, pointed it at the intersection below, and let Claude watch the road Claude tags every car, motorbike and pedestrian in real time, 653 in five minutes, and flags anyone over the limit The moment a car speeds, Claude clips the video, reads the license plate, matches the owner, and emails the fine on its own A normal radar takes one photo and misses half the time. Claude records full video, so there is nothing to dispute, and the fines go out with no operator He walked into the district office with a flash drive and asked for 10 minutes. he left with a contract Every Claude config he used is in the article

Fokki

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Some friends wonder how we spend our time here in the war in Gaza with our children!! As for me, I dedicate my time to making my sons Imad and Adam happy — for example, I built a swing inside the tent so we could play and share a little joy together. I always try to protect them, make them smile, and help them forget the terrifying sounds of the bombing and the fear. I wish I could fly them away to a safe and beautiful future 💔 If you’d like to be part of our survival, you can help by donating💕:

Some friends wonder how we spend our time here in the war in Gaza with our children!! As for me, I dedicate my time to making my sons Imad and Adam happy — for example, I built a swing inside the tent so we could play and share a little joy together. I always try to protect them, make them smile, and help them forget the terrifying sounds of the bombing and the fear. I wish I could fly them away to a safe and beautiful future 💔 If you’d like to be part of our survival, you can help by donating💕:

mohammed hussein~Gaza 🇵🇸

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OSINT: This bandit named Auutadawa, in the first frame showed a location likely to be in North Central with his colleagues, then the frame 2 video showed he has been presented with a new bike from the victims family, and interesting catch was the two victim seems to be in background of the video as seen one of them limping. In the third video it shows ransom payment received ranging to millions of naira. It is important security agencies keep track of these things.

OSINT: This bandit named Auutadawa, in the first frame showed a location likely to be in North Central with his colleagues, then the frame 2 video showed he has been presented with a new bike from the victims family, and interesting catch was the two victim seems to be in background of the video as seen one of them limping. In the third video it shows ransom payment received ranging to millions of naira. It is important security agencies keep track of these things.

Mobilisingnigerians™

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The complexity of an AI driven robot that needs to meet GMP standards & learn complex steps of targeted cell manufacture. A marvel and the first robot of its kind in the world. Can’t wait till we lift off the first patient’s NK cells in LA from NANT LEONARDO. Unstoppable scale.

The complexity of an AI driven robot that needs to meet GMP standards & learn complex steps of targeted cell manufacture. A marvel and the first robot of its kind in the world. Can’t wait till we lift off the first patient’s NK cells in LA from NANT LEONARDO. Unstoppable scale.

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Prince William has been in Bournemouth with a group of delegates from around the world to show them the work of his Homewards initiative to end homelessness. He delighted Lily Childs, 12, who asked him for a selfie when he arrived by returning to take one after the engagement

Prince William has been in Bournemouth with a group of delegates from around the world to show them the work of his Homewards initiative to end homelessness. He delighted Lily Childs, 12, who asked him for a selfie when he arrived by returning to take one after the engagement

Rebecca English

61,604 görüntüleme • 6 ay önce

For 50 years, science told us genes were fixed our destiny written at birth. In 2003, the Human Genome Project quietly shattered that belief. What it revealed instead is a discovery in epigenetics that should be taught in every school on Earth: 1. The old model said: you have a gene for anxiety. A gene for depression. A gene for heart disease. A gene for early death. You inherited it from your parents. Your children will inherit it from you. Nothing you do changes it. The Human Genome Project; a 13-year, $3 billion study was supposed to confirm this. Instead, it revealed something no one expected: Humans have roughly 23,000 genes. Less than a roundworm. The "genetic destiny" model collapsed overnight. 2. What emerged in its place was the field of epigenetics, the science of how your environment controls your DNA. The discovery: genes do not determine your life. Signals from the environment turn genes on and off. And the environment that matters most? Your internal environment. Your thoughts. Your emotions. Science now shows that the emotion you sustain for as little as 15 to 20 minutes a day can signal your immune cells to begin producing new proteins. Your emotional state is writing instructions to your DNA. Right now. 3. In Dr. Joe Dispenza's workshops, students measured for gene expression changes after just 4 days. Significant shifts detected. IgA the immune marker increased measurably. New proteins. New enzymes. New hormones. From thought and emotion alone. No drugs. No surgery. No supplements just the mind-body connection in action. 4. This means: The disease your parent had is not your sentence. The anxiety that runs in your family is a pattern not a prophecy. The body you inherited is not the body you're confined to. You are not a victim of your biology. You are the author of it. Every day you choose the emotional signals you flood your cells with and those signals are rewriting the program. 5. The most powerful pharmacy in existence is inside you. You don't need a prescription for it. You need a practice.

For 50 years, science told us genes were fixed our destiny written at birth. In 2003, the Human Genome Project quietly shattered that belief. What it revealed instead is a discovery in epigenetics that should be taught in every school on Earth: 1. The old model said: you have a gene for anxiety. A gene for depression. A gene for heart disease. A gene for early death. You inherited it from your parents. Your children will inherit it from you. Nothing you do changes it. The Human Genome Project; a 13-year, $3 billion study was supposed to confirm this. Instead, it revealed something no one expected: Humans have roughly 23,000 genes. Less than a roundworm. The "genetic destiny" model collapsed overnight. 2. What emerged in its place was the field of epigenetics, the science of how your environment controls your DNA. The discovery: genes do not determine your life. Signals from the environment turn genes on and off. And the environment that matters most? Your internal environment. Your thoughts. Your emotions. Science now shows that the emotion you sustain for as little as 15 to 20 minutes a day can signal your immune cells to begin producing new proteins. Your emotional state is writing instructions to your DNA. Right now. 3. In Dr. Joe Dispenza's workshops, students measured for gene expression changes after just 4 days. Significant shifts detected. IgA the immune marker increased measurably. New proteins. New enzymes. New hormones. From thought and emotion alone. No drugs. No surgery. No supplements just the mind-body connection in action. 4. This means: The disease your parent had is not your sentence. The anxiety that runs in your family is a pattern not a prophecy. The body you inherited is not the body you're confined to. You are not a victim of your biology. You are the author of it. Every day you choose the emotional signals you flood your cells with and those signals are rewriting the program. 5. The most powerful pharmacy in existence is inside you. You don't need a prescription for it. You need a practice.

🧬Maxpein🧬

168,719 görüntüleme • 24 gün önce

👀🇺🇦 In August last year, during Budanov’s visit, together with soldiers of GUR, to one of the “Boyko towers” in the Black Sea, the Russians noticed them and launched drones at it. He decided to jump from the height of the Crimea-1 gas production platform into the water with everyone else.

👀🇺🇦 In August last year, during Budanov’s visit, together with soldiers of GUR, to one of the “Boyko towers” in the Black Sea, the Russians noticed them and launched drones at it. He decided to jump from the height of the Crimea-1 gas production platform into the water with everyone else.

MAKS 25 🇺🇦👀

240,877 görüntüleme • 5 ay önce

A single E. coli cell, placed on a dish, will become 70 billion cells in just 12 hours. That’s exponential growth. But a new preprint shows that it's possible to engineer E. coli to grow linearly instead, where only one daughter cell continues dividing and the other stops. First, some context. In nature, there is a bacterium called Mycobacterium smegmatis (initially discovered in 1884 in ulcers scraped from syphilis patients.) M. smegmatis is weird because it divides asymmetrically. These cells grow only from one end, and all their cell wall biosynthesis machinery is located on that one end. So when the cell divides, one daughter gets this machinery and the other gets nothing. The daughter that gets the machinery can keep dividing immediately, but the other daughter has to remake all that machinery from scratch, so its growth is delayed. E. coli doesn’t grow like this. When it divides, it pinches in the middle and splits everything evenly. Enzymes, metabolites, and proteins get partitioned more or less randomly between the two daughters. For the new preprint, though, researchers engineered E. coli to behave more like M. smegmatis. Here is how they did it: First, they deleted a gene called cyaA, which encodes an enzyme (adenylate cyclase) that makes a molecule called cAMP. cAMP is SUPER IMPORTANT! It is a nutrient sensor that instructs E. coli to switch on genes that help it digest non-glucose carbon sources when glucose is scarce. Without cAMP, E. coli cells growing on alternative carbon sources will starve; they won’t know how to eat the food. Next, they added back a “split” version of the cyaA gene into the cells. In other words, they split the gene in two so that each half of the enzyme is made separately. Cells can only make cAMP, and thus eat non-glucose carbon sources, if these two halves come together. To facilitate that “coming together,” the researchers also fused the split cyaA proteins to sticky proteins that clump together, and to a fluorescent protein (to make it easy to track these molecules in the cell.) So now some interesting things start to happen if you grow E. coli on a growth medium lacking glucose. As the cell grows, its cyaA “halves” start clumping together into a giant ball. Inside the aggregate, the two enzyme halves come together and make cAMP. And when the cell gets big enough and divides, the clump of cyaA RANDOMLY goes to either daughter cell #1 or #2. The daughter that gets the aggregate (called PA+ in this paper) can keep dividing. The daughter that doesn’t (PA–) cannot. It still grows a few times — about four divisions — because it inherits some leftover cAMP from its mother. But after that, the metabolite is diluted away, and the cell stops growing. PA+ cells went through about 23 divisions on average before their aggregate decayed. And the population of cells, as a whole, grew linearly. This paper is cool because there are many applications where exponential growth is too unpredictable and, perhaps, unsafe. If you want to engineer bacteria to deliver drugs, clean up waste, or live in the gut, you don’t want them to double uncontrollably. This paper shows you can make them expand in a controlled, linear way. Alas, mutations could break this whole engineered system. A mutation that restores cyaA, for example, would give cells a new way to make cAMP. Mutations that make the aggregates split between daughters would break the asymmetry, too. But still, I really enjoy proof-of-concept engineering papers like this.

A single E. coli cell, placed on a dish, will become 70 billion cells in just 12 hours. That’s exponential growth. But a new preprint shows that it's possible to engineer E. coli to grow linearly instead, where only one daughter cell continues dividing and the other stops. First, some context. In nature, there is a bacterium called Mycobacterium smegmatis (initially discovered in 1884 in ulcers scraped from syphilis patients.) M. smegmatis is weird because it divides asymmetrically. These cells grow only from one end, and all their cell wall biosynthesis machinery is located on that one end. So when the cell divides, one daughter gets this machinery and the other gets nothing. The daughter that gets the machinery can keep dividing immediately, but the other daughter has to remake all that machinery from scratch, so its growth is delayed. E. coli doesn’t grow like this. When it divides, it pinches in the middle and splits everything evenly. Enzymes, metabolites, and proteins get partitioned more or less randomly between the two daughters. For the new preprint, though, researchers engineered E. coli to behave more like M. smegmatis. Here is how they did it: First, they deleted a gene called cyaA, which encodes an enzyme (adenylate cyclase) that makes a molecule called cAMP. cAMP is SUPER IMPORTANT! It is a nutrient sensor that instructs E. coli to switch on genes that help it digest non-glucose carbon sources when glucose is scarce. Without cAMP, E. coli cells growing on alternative carbon sources will starve; they won’t know how to eat the food. Next, they added back a “split” version of the cyaA gene into the cells. In other words, they split the gene in two so that each half of the enzyme is made separately. Cells can only make cAMP, and thus eat non-glucose carbon sources, if these two halves come together. To facilitate that “coming together,” the researchers also fused the split cyaA proteins to sticky proteins that clump together, and to a fluorescent protein (to make it easy to track these molecules in the cell.) So now some interesting things start to happen if you grow E. coli on a growth medium lacking glucose. As the cell grows, its cyaA “halves” start clumping together into a giant ball. Inside the aggregate, the two enzyme halves come together and make cAMP. And when the cell gets big enough and divides, the clump of cyaA RANDOMLY goes to either daughter cell #1 or #2. The daughter that gets the aggregate (called PA+ in this paper) can keep dividing. The daughter that doesn’t (PA–) cannot. It still grows a few times — about four divisions — because it inherits some leftover cAMP from its mother. But after that, the metabolite is diluted away, and the cell stops growing. PA+ cells went through about 23 divisions on average before their aggregate decayed. And the population of cells, as a whole, grew linearly. This paper is cool because there are many applications where exponential growth is too unpredictable and, perhaps, unsafe. If you want to engineer bacteria to deliver drugs, clean up waste, or live in the gut, you don’t want them to double uncontrollably. This paper shows you can make them expand in a controlled, linear way. Alas, mutations could break this whole engineered system. A mutation that restores cyaA, for example, would give cells a new way to make cAMP. Mutations that make the aggregates split between daughters would break the asymmetry, too. But still, I really enjoy proof-of-concept engineering papers like this.

Niko McCarty.

58,094 görüntüleme • 1 yıl önce