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🦠BIG #rotavirus infection🦠 I’ve probably infected more cells before using roller bottles. But these are the biggest plates (500cm2) I have every used for an infection. 🎲 🎲 come on cells, give me some protein!

Hyser Lab

1,818 subscribers

12,189 次观看 • 3 年前 •via X (Twitter)

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This is an interesting one: 2 rounds of antibiotics given to a patient for a chest infection. Patient still unwell. I wonder how much microbiology and pathology PAs have done in order to pick up what sort of chest infection this may have been in the first place (so to give the right antibiotics if needed) Wonder if this was a bacterial infection in the first place? Intrigued to understand what PA next steps would be? Had the patient seen the GP the second time? Interesting take on educating the public and interesting method by being pretty passive aggressive - unsure if this is the technique to be used. Glad that they are educating on antibiotic resistance though. I wonder how much this is happening causing increased activity, increased wait times and increased activity (unnecessarily)

This is an interesting one: 2 rounds of antibiotics given to a patient for a chest infection. Patient still unwell. I wonder how much microbiology and pathology PAs have done in order to pick up what sort of chest infection this may have been in the first place (so to give the right antibiotics if needed) Wonder if this was a bacterial infection in the first place? Intrigued to understand what PA next steps would be? Had the patient seen the GP the second time? Interesting take on educating the public and interesting method by being pretty passive aggressive - unsure if this is the technique to be used. Glad that they are educating on antibiotic resistance though. I wonder how much this is happening causing increased activity, increased wait times and increased activity (unnecessarily)

Dr Sandeep Bansal

21,451 次观看 • 1 年前

There's a bacteriophage that turns bacteria into “liquid crystals.” Specifically, Pseudomonas aeruginosa bacteria make Pf phages, which are rod-shaped, negatively-charged, and measure about 2 micrometers in length (roughly the length of an E. coli cell). These phages leave the cells and enter their surroundings. There, they mix with polymers, also secreted by the cells, to form a crystalline matrix. Surprisingly, this is good for the cells. Although the phages kill some of them, it also makes their biofilms stickier and able to withstand certain antibiotics. These bacteria + phages are prevalent in cystic fibrosis patients; they've formed a sort of symbiotic relationship. The Pf phages are made from thousands of repeating copies of a coat protein, called CoaB, which wraps around a single-stranded, circular DNA genome. These genes are integrated directly on the bacterial chromosome. The bacteria “turn on” these phage genes when placed in a viscous environment with low oxygen levels. This is like a trigger to start forming a biofilm. And the cells make a lot of phages; about 100 billion per milliliter. These liquid crystals form because of a physics principle called “depletion attraction.” If you just mix a bunch of loose or flexible polymers together (such as long carbon chains) they will not form a liquid crystal. But if you mix stiff rods (the phages) with loose polymers at a high enough concentration, the polymers will force the phages close together to create a material that flows like a liquid despite being ordered like a crystal. See the video below. These liquid crystal biofilms are hard to get rid of. The negatively-charged phages block many antibiotics (like aminoglycosides, which are positively-charged) from entering cells. Liquid crystals also retain water, so these biofilms can survive on drier surfaces. I first heard about this from Malmesbury’s excellent newsletter, called “Telescopic Turnip.”

There's a bacteriophage that turns bacteria into “liquid crystals.” Specifically, Pseudomonas aeruginosa bacteria make Pf phages, which are rod-shaped, negatively-charged, and measure about 2 micrometers in length (roughly the length of an E. coli cell). These phages leave the cells and enter their surroundings. There, they mix with polymers, also secreted by the cells, to form a crystalline matrix. Surprisingly, this is good for the cells. Although the phages kill some of them, it also makes their biofilms stickier and able to withstand certain antibiotics. These bacteria + phages are prevalent in cystic fibrosis patients; they've formed a sort of symbiotic relationship. The Pf phages are made from thousands of repeating copies of a coat protein, called CoaB, which wraps around a single-stranded, circular DNA genome. These genes are integrated directly on the bacterial chromosome. The bacteria “turn on” these phage genes when placed in a viscous environment with low oxygen levels. This is like a trigger to start forming a biofilm. And the cells make a lot of phages; about 100 billion per milliliter. These liquid crystals form because of a physics principle called “depletion attraction.” If you just mix a bunch of loose or flexible polymers together (such as long carbon chains) they will not form a liquid crystal. But if you mix stiff rods (the phages) with loose polymers at a high enough concentration, the polymers will force the phages close together to create a material that flows like a liquid despite being ordered like a crystal. See the video below. These liquid crystal biofilms are hard to get rid of. The negatively-charged phages block many antibiotics (like aminoglycosides, which are positively-charged) from entering cells. Liquid crystals also retain water, so these biofilms can survive on drier surfaces. I first heard about this from Malmesbury’s excellent newsletter, called “Telescopic Turnip.”

Niko McCarty.

50,029 次观看 • 6 个月前

Used my friend's iPad 10th Gen and the animations are phenomenal as expected but that 60hz is 🤢 idk how people find this acceptable especially on the iPad Air. Idk why but 60hz has become far more intolerable for me since I started using LineageOS on my Xiaomi Pad 5. It's multiple decades ahead in smoothness. Even that unoptimised jittery ass HyperOS for Pad was better on 120hz but not as much. Also what are these random bugs on the iPad? It's running iPadOS 17.5.1 but those bugs are random af.

Used my friend's iPad 10th Gen and the animations are phenomenal as expected but that 60hz is 🤢 idk how people find this acceptable especially on the iPad Air. Idk why but 60hz has become far more intolerable for me since I started using LineageOS on my Xiaomi Pad 5. It's multiple decades ahead in smoothness. Even that unoptimised jittery ass HyperOS for Pad was better on 120hz but not as much. Also what are these random bugs on the iPad? It's running iPadOS 17.5.1 but those bugs are random af.

Uncle Yoyo 🍉

28,156 次观看 • 1 年前

I can’t sleep. Someone tell me where did we get it wrong? How can this mammoth crowd be in CBD, for an Occupy StateHouse protest, but walk in the opposite direction? Something’s not right. I know we scored huge but that wasn’t the plan. We’ve been massively infiltrated and the people upfront in these marches are co-opted. So how do we get the masses to not follow one massive train and instead split into various cells with independence of thought? This is the big question. #OccupyStatehouse2025 #OccupyUntilVictory #SiriNiNumbers

I can’t sleep. Someone tell me where did we get it wrong? How can this mammoth crowd be in CBD, for an Occupy StateHouse protest, but walk in the opposite direction? Something’s not right. I know we scored huge but that wasn’t the plan. We’ve been massively infiltrated and the people upfront in these marches are co-opted. So how do we get the masses to not follow one massive train and instead split into various cells with independence of thought? This is the big question. #OccupyStatehouse2025 #OccupyUntilVictory #SiriNiNumbers

Francis Gaitho

72,233 次观看 • 1 年前

🚨 BREAKING: Human cells may behave far more like quantum systems than we ever imagined. New research suggests biological cells process information in ways surprisingly similar to quantum computers. Your body already runs on electrical signaling. But scientists are now exploring whether quantum-like effects help coordinate biological processes at microscopic scales. That changes the question completely: Maybe consciousness, memory, and cellular communication are not just chemical reactions… but emergent information fields operating across complex electrical networks. Nature may have discovered quantum optimization billions of years before humans built quantum computers. If true, biology itself could become the blueprint for the next generation of computing. We might not be building machines to mimic humans. We may be rediscovering the physics life has been using all along. Follow for more breakthroughs where physics, biology, and computation collide.

🚨 BREAKING: Human cells may behave far more like quantum systems than we ever imagined. New research suggests biological cells process information in ways surprisingly similar to quantum computers. Your body already runs on electrical signaling. But scientists are now exploring whether quantum-like effects help coordinate biological processes at microscopic scales. That changes the question completely: Maybe consciousness, memory, and cellular communication are not just chemical reactions… but emergent information fields operating across complex electrical networks. Nature may have discovered quantum optimization billions of years before humans built quantum computers. If true, biology itself could become the blueprint for the next generation of computing. We might not be building machines to mimic humans. We may be rediscovering the physics life has been using all along. Follow for more breakthroughs where physics, biology, and computation collide.

TheNewPhysics

12,200 次观看 • 2 个月前

Over the last ~year I’ve definitely doubled down on my own health and wellness. Some days I go hard and others are more chill. But every single day has one objective and that’s to move. Doesn’t have to be a 🔨 session in the gym or on the bike. Could just be a 20 minute walk around your neighborhood or park. Thanks KetoneIQ for keeping me movin’. #sponsored #ketoneiq

Over the last ~year I’ve definitely doubled down on my own health and wellness. Some days I go hard and others are more chill. But every single day has one objective and that’s to move. Doesn’t have to be a 🔨 session in the gym or on the bike. Could just be a 20 minute walk around your neighborhood or park. Thanks KetoneIQ for keeping me movin’. #sponsored #ketoneiq

Lance Armstrong

194,694 次观看 • 1 年前

Its sad for me to see Wout Poels have one of his biggest moments, winning a stage Tour de France™, which is an almost impossible task. I would really appreciate for him or any winner to have more than 2 seconds of TV time, so he can show his son what he had achieved. The GC guys are shown everyday. Please, I ask kindly France Télévisions give these guys and their teams more exposure that they deserve...

Its sad for me to see Wout Poels have one of his biggest moments, winning a stage Tour de France™, which is an almost impossible task. I would really appreciate for him or any winner to have more than 2 seconds of TV time, so he can show his son what he had achieved. The GC guys are shown everyday. Please, I ask kindly France Télévisions give these guys and their teams more exposure that they deserve...

Adam Hansen

856,126 次观看 • 3 年前

A single E. coli cell, placed on a dish, will become 70 billion cells in just 12 hours. That’s exponential growth. But a new preprint shows that it's possible to engineer E. coli to grow linearly instead, where only one daughter cell continues dividing and the other stops. First, some context. In nature, there is a bacterium called Mycobacterium smegmatis (initially discovered in 1884 in ulcers scraped from syphilis patients.) M. smegmatis is weird because it divides asymmetrically. These cells grow only from one end, and all their cell wall biosynthesis machinery is located on that one end. So when the cell divides, one daughter gets this machinery and the other gets nothing. The daughter that gets the machinery can keep dividing immediately, but the other daughter has to remake all that machinery from scratch, so its growth is delayed. E. coli doesn’t grow like this. When it divides, it pinches in the middle and splits everything evenly. Enzymes, metabolites, and proteins get partitioned more or less randomly between the two daughters. For the new preprint, though, researchers engineered E. coli to behave more like M. smegmatis. Here is how they did it: First, they deleted a gene called cyaA, which encodes an enzyme (adenylate cyclase) that makes a molecule called cAMP. cAMP is SUPER IMPORTANT! It is a nutrient sensor that instructs E. coli to switch on genes that help it digest non-glucose carbon sources when glucose is scarce. Without cAMP, E. coli cells growing on alternative carbon sources will starve; they won’t know how to eat the food. Next, they added back a “split” version of the cyaA gene into the cells. In other words, they split the gene in two so that each half of the enzyme is made separately. Cells can only make cAMP, and thus eat non-glucose carbon sources, if these two halves come together. To facilitate that “coming together,” the researchers also fused the split cyaA proteins to sticky proteins that clump together, and to a fluorescent protein (to make it easy to track these molecules in the cell.) So now some interesting things start to happen if you grow E. coli on a growth medium lacking glucose. As the cell grows, its cyaA “halves” start clumping together into a giant ball. Inside the aggregate, the two enzyme halves come together and make cAMP. And when the cell gets big enough and divides, the clump of cyaA RANDOMLY goes to either daughter cell #1 or #2. The daughter that gets the aggregate (called PA+ in this paper) can keep dividing. The daughter that doesn’t (PA–) cannot. It still grows a few times — about four divisions — because it inherits some leftover cAMP from its mother. But after that, the metabolite is diluted away, and the cell stops growing. PA+ cells went through about 23 divisions on average before their aggregate decayed. And the population of cells, as a whole, grew linearly. This paper is cool because there are many applications where exponential growth is too unpredictable and, perhaps, unsafe. If you want to engineer bacteria to deliver drugs, clean up waste, or live in the gut, you don’t want them to double uncontrollably. This paper shows you can make them expand in a controlled, linear way. Alas, mutations could break this whole engineered system. A mutation that restores cyaA, for example, would give cells a new way to make cAMP. Mutations that make the aggregates split between daughters would break the asymmetry, too. But still, I really enjoy proof-of-concept engineering papers like this.

A single E. coli cell, placed on a dish, will become 70 billion cells in just 12 hours. That’s exponential growth. But a new preprint shows that it's possible to engineer E. coli to grow linearly instead, where only one daughter cell continues dividing and the other stops. First, some context. In nature, there is a bacterium called Mycobacterium smegmatis (initially discovered in 1884 in ulcers scraped from syphilis patients.) M. smegmatis is weird because it divides asymmetrically. These cells grow only from one end, and all their cell wall biosynthesis machinery is located on that one end. So when the cell divides, one daughter gets this machinery and the other gets nothing. The daughter that gets the machinery can keep dividing immediately, but the other daughter has to remake all that machinery from scratch, so its growth is delayed. E. coli doesn’t grow like this. When it divides, it pinches in the middle and splits everything evenly. Enzymes, metabolites, and proteins get partitioned more or less randomly between the two daughters. For the new preprint, though, researchers engineered E. coli to behave more like M. smegmatis. Here is how they did it: First, they deleted a gene called cyaA, which encodes an enzyme (adenylate cyclase) that makes a molecule called cAMP. cAMP is SUPER IMPORTANT! It is a nutrient sensor that instructs E. coli to switch on genes that help it digest non-glucose carbon sources when glucose is scarce. Without cAMP, E. coli cells growing on alternative carbon sources will starve; they won’t know how to eat the food. Next, they added back a “split” version of the cyaA gene into the cells. In other words, they split the gene in two so that each half of the enzyme is made separately. Cells can only make cAMP, and thus eat non-glucose carbon sources, if these two halves come together. To facilitate that “coming together,” the researchers also fused the split cyaA proteins to sticky proteins that clump together, and to a fluorescent protein (to make it easy to track these molecules in the cell.) So now some interesting things start to happen if you grow E. coli on a growth medium lacking glucose. As the cell grows, its cyaA “halves” start clumping together into a giant ball. Inside the aggregate, the two enzyme halves come together and make cAMP. And when the cell gets big enough and divides, the clump of cyaA RANDOMLY goes to either daughter cell #1 or #2. The daughter that gets the aggregate (called PA+ in this paper) can keep dividing. The daughter that doesn’t (PA–) cannot. It still grows a few times — about four divisions — because it inherits some leftover cAMP from its mother. But after that, the metabolite is diluted away, and the cell stops growing. PA+ cells went through about 23 divisions on average before their aggregate decayed. And the population of cells, as a whole, grew linearly. This paper is cool because there are many applications where exponential growth is too unpredictable and, perhaps, unsafe. If you want to engineer bacteria to deliver drugs, clean up waste, or live in the gut, you don’t want them to double uncontrollably. This paper shows you can make them expand in a controlled, linear way. Alas, mutations could break this whole engineered system. A mutation that restores cyaA, for example, would give cells a new way to make cAMP. Mutations that make the aggregates split between daughters would break the asymmetry, too. But still, I really enjoy proof-of-concept engineering papers like this.

Niko McCarty.

58,019 次观看 • 10 个月前

for me, rust needs a deeper and more complex combat change. before anyone says to "just play a different way, change your playstyle", for the last year and a half i have done nothing but constantly change how i play and as well how i produce content, to see if the issue lies with me. but with every month that goes by it just gets harder and harder to hold onto the passion i once had for the game, i dont care about wanting to be one of the best anymore, or spending 12 hours a day training because it just doesnt satisfy me the way it used to and i hate that. a lot of people are probably gonna say to just quit rust or to move on- which isnt bad advice but i think i'd rather stay delusional and hope another combat update happens soon. better times are coming brothers and sisters i believe in it 👍

for me, rust needs a deeper and more complex combat change. before anyone says to "just play a different way, change your playstyle", for the last year and a half i have done nothing but constantly change how i play and as well how i produce content, to see if the issue lies with me. but with every month that goes by it just gets harder and harder to hold onto the passion i once had for the game, i dont care about wanting to be one of the best anymore, or spending 12 hours a day training because it just doesnt satisfy me the way it used to and i hate that. a lot of people are probably gonna say to just quit rust or to move on- which isnt bad advice but i think i'd rather stay delusional and hope another combat update happens soon. better times are coming brothers and sisters i believe in it 👍

Sinks

22,427 次观看 • 2 年前

After 5 years in Solana, I honestly can’t believe we’re here selling out arenas for a crypto event. I’ve never seen anything like this. The Breakpoint venue is insane. The pop-ups are so clean and creative. All our favorite Solana brands are here. In my whole web3 journey, I’ve never been to an event like this. The energy is unreal. It really feels like the biggest event in crypto. Everyone is here. All my Solana friends, all the builders, every project you can think of. The whole ecosystem pulled up. It’s surreal to see how far we’ve come, I truly mean it. I felt a bit emotional looking at everything today. We have more users, more apps, more tech, more institutions, and more real usage than ever before. You cannot be bearish on Solana after experiencing this. This is the chain that’s going to run global finance. Everything is happening here. Solana is the everything chain.

After 5 years in Solana, I honestly can’t believe we’re here selling out arenas for a crypto event. I’ve never seen anything like this. The Breakpoint venue is insane. The pop-ups are so clean and creative. All our favorite Solana brands are here. In my whole web3 journey, I’ve never been to an event like this. The energy is unreal. It really feels like the biggest event in crypto. Everyone is here. All my Solana friends, all the builders, every project you can think of. The whole ecosystem pulled up. It’s surreal to see how far we’ve come, I truly mean it. I felt a bit emotional looking at everything today. We have more users, more apps, more tech, more institutions, and more real usage than ever before. You cannot be bearish on Solana after experiencing this. This is the chain that’s going to run global finance. Everything is happening here. Solana is the everything chain.

Solana Sensei

21,718 次观看 • 7 个月前

Today I received my 4th infusion of SGF (Stem Cell Growth Factors) at Edogawa Hospital in Tokyo, Japan. What is SGF? 🦷 SGF is derived from the dental pulp of children's naturally shed baby teeth. No stem cells are injected. Instead, the infusion contains the signaling molecules, growth factors, cytokines, and regenerative proteins that stem cells naturally produce. These biological messengers help coordinate communication between cells and are believed to support tissue repair, immune regulation, blood vessel health, and nerve regeneration. These growth factors are small enough to cross the blood brain barrier and reach the brain and nervous system directly. For someone like me with confirmed neuroinflammation, white matter atrophy, and small fiber neuropathy destroying my nerves from the inside out, the goal is remyelination. Rebuilding the insulation around my damaged nerve fibers.💯 Researchers have studied SHED (Stem Cells from Human Exfoliated Deciduous Teeth) for their regenerative potential in areas such as nerve repair, neuroinflammation, tissue healing, and age-related degeneration. Some people have nicknamed therapies like this the "Fountain of Youth" because the goal is not to replace damaged tissue, but to activate the body's own repair and regeneration pathways. Whether that nickname is deserved remains to be seen, but the science behind cellular signaling and regeneration is fascinating. Japan has become a global leader in regenerative medicine and allows access to therapies that are not currently available in the United States under its regenerative medicine framework. As Patient #27 in the McCairn–Edogawa Protocol, I am grateful to have the opportunity to experience this emerging science firsthand. 🧬🦷🇯🇵 #SGF #SHED #RegenerativeMedicine #StemCellScience #Neuroinflammation #SmallFiberNeuropathy #EdogawaHospital #Tokyo #MedicalInnovation

Today I received my 4th infusion of SGF (Stem Cell Growth Factors) at Edogawa Hospital in Tokyo, Japan. What is SGF? 🦷 SGF is derived from the dental pulp of children's naturally shed baby teeth. No stem cells are injected. Instead, the infusion contains the signaling molecules, growth factors, cytokines, and regenerative proteins that stem cells naturally produce. These biological messengers help coordinate communication between cells and are believed to support tissue repair, immune regulation, blood vessel health, and nerve regeneration. These growth factors are small enough to cross the blood brain barrier and reach the brain and nervous system directly. For someone like me with confirmed neuroinflammation, white matter atrophy, and small fiber neuropathy destroying my nerves from the inside out, the goal is remyelination. Rebuilding the insulation around my damaged nerve fibers.💯 Researchers have studied SHED (Stem Cells from Human Exfoliated Deciduous Teeth) for their regenerative potential in areas such as nerve repair, neuroinflammation, tissue healing, and age-related degeneration. Some people have nicknamed therapies like this the "Fountain of Youth" because the goal is not to replace damaged tissue, but to activate the body's own repair and regeneration pathways. Whether that nickname is deserved remains to be seen, but the science behind cellular signaling and regeneration is fascinating. Japan has become a global leader in regenerative medicine and allows access to therapies that are not currently available in the United States under its regenerative medicine framework. As Patient #27 in the McCairn–Edogawa Protocol, I am grateful to have the opportunity to experience this emerging science firsthand. 🧬🦷🇯🇵 #SGF #SHED #RegenerativeMedicine #StemCellScience #Neuroinflammation #SmallFiberNeuropathy #EdogawaHospital #Tokyo #MedicalInnovation

Heather C

28,961 次观看 • 1 个月前

Kitten update #8: The kitties are back home! Everyone is getting very strong and active now. I forgot to take their weights this morning so I'll have to do it before the next feeding, but I'm confident everyone is continuing to gain. We started adding in some wet food today. I mixed up a slurry with some of the formula and stuck it in a syringe with a nipple on it. Nobody took to it right away except gray tuxedo, who loved it, but everyone else got some squirted in while they were chewing on the milk nipple and then they loved it. Well, except for dark tortie, which is a little ironic since she is by far the biggest little monster of the litter, but I guess she's done so well on the formula she's not interested in making a change yet. Butts are all looking SO much better now that I stopped using the wet wipes. However, we have now started to have some concerns about little to no pooping. Dark tortie hasn't pooped for 24 hours so I eliminated the yogurt from the formula and am crossing my fingers for some action before the end of the day or back to the vet she goes. I don't know how long they'll let me play with their tummies like this but it sure is fun for now!

Kitten update #8: The kitties are back home! Everyone is getting very strong and active now. I forgot to take their weights this morning so I'll have to do it before the next feeding, but I'm confident everyone is continuing to gain. We started adding in some wet food today. I mixed up a slurry with some of the formula and stuck it in a syringe with a nipple on it. Nobody took to it right away except gray tuxedo, who loved it, but everyone else got some squirted in while they were chewing on the milk nipple and then they loved it. Well, except for dark tortie, which is a little ironic since she is by far the biggest little monster of the litter, but I guess she's done so well on the formula she's not interested in making a change yet. Butts are all looking SO much better now that I stopped using the wet wipes. However, we have now started to have some concerns about little to no pooping. Dark tortie hasn't pooped for 24 hours so I eliminated the yogurt from the formula and am crossing my fingers for some action before the end of the day or back to the vet she goes. I don't know how long they'll let me play with their tummies like this but it sure is fun for now!

Andrea Burkhart 🐟🐟🐟🐟🏴‍☠️

27,090 次观看 • 10 个月前

I am very happy to report we have scanned the last page of the Howard W. Sams Photofact books for AI training. Here is the video of the final page of 1000s. No, not to repair TVs and Radios. But why? Many reasons. One is to capture the logic and thinking processes of an ancient epoch. This book series alone will align ANY AI platform to higher abilities because of the thinking used. Another is to understand what analog technology really is in a modern concept as it informs many functions completely unrelated to electronics. Thus I now am going to plan on a preservation process for these books that should allow them to be available for the next few 100 years or perhaps 1000 years. They are a masterpiece of very high protein data. In fact the highest protein data I have discovered, each square inch is valuable. I will write a series of articles on some landmark discoveries Ai have made in these books. More soon. And once again thank you for this donation. Thank you for thinking of our future through our past. Many say they will “help me out” some very smart and very wealthy, this guy came through. Gratitude.

I am very happy to report we have scanned the last page of the Howard W. Sams Photofact books for AI training. Here is the video of the final page of 1000s. No, not to repair TVs and Radios. But why? Many reasons. One is to capture the logic and thinking processes of an ancient epoch. This book series alone will align ANY AI platform to higher abilities because of the thinking used. Another is to understand what analog technology really is in a modern concept as it informs many functions completely unrelated to electronics. Thus I now am going to plan on a preservation process for these books that should allow them to be available for the next few 100 years or perhaps 1000 years. They are a masterpiece of very high protein data. In fact the highest protein data I have discovered, each square inch is valuable. I will write a series of articles on some landmark discoveries Ai have made in these books. More soon. And once again thank you for this donation. Thank you for thinking of our future through our past. Many say they will “help me out” some very smart and very wealthy, this guy came through. Gratitude.

Brian Roemmele

55,922 次观看 • 2 个月前

I've been sitting on this (there are more) for just the right moment. This was at the Verso loft for a fundraiser for the Zohran/Phara/Samelys/Jabari slate. Everyone was amazing. Dania and I were blown away. But we also realized: no was going to give. We're not rich but we believe in the cause and are old enough to have lived through so much failure. So we forked over the big first donation. Mamdani today is making it look worth every penny. I suffer no illusions about what is coming but today is a day to feel good...

I've been sitting on this (there are more) for just the right moment. This was at the Verso loft for a fundraiser for the Zohran/Phara/Samelys/Jabari slate. Everyone was amazing. Dania and I were blown away. But we also realized: no was going to give. We're not rich but we believe in the cause and are old enough to have lived through so much failure. So we forked over the big first donation. Mamdani today is making it look worth every penny. I suffer no illusions about what is coming but today is a day to feel good...

Ajay Singh Chaudhary

27,770 次观看 • 8 个月前

❗️This video has gone ridiculously viral on every platform❗️I know a lot of coaches follow me on Twitter & I am keen for your thoughts on this.. but before you comment, please watch the full version first➡️ As a youth player, we did this drill A lot at Wrexham under the great Joey Jones. To introduce the feel of a tackle & breaking that fear barrier that most kids have these days. Keen for your thoughts, I will know from some replies that you haven’t watched the full version … #JonerFootball #Tackle

❗️This video has gone ridiculously viral on every platform❗️I know a lot of coaches follow me on Twitter & I am keen for your thoughts on this.. but before you comment, please watch the full version first➡️ As a youth player, we did this drill A lot at Wrexham under the great Joey Jones. To introduce the feel of a tackle & breaking that fear barrier that most kids have these days. Keen for your thoughts, I will know from some replies that you haven’t watched the full version … #JonerFootball #Tackle

Joner Football

359,574 次观看 • 6 个月前

Some microbes carry a protein, called SNIPE, that "chops up" phage DNA as it's being injected into the cell. This is a new mechanism for phage defense! CRISPR–Cas and restriction enzymes also evolved to fight against phages, but they work by recognizing sequences. SNIPE works, instead, by sensing "touch." SNIPE is a protein with about 500 amino acids. After it's made by the ribosome, it latches onto ManYZ, two proteins which sit on the cell's inner membrane. (ManYZ is an importer; it brings mannose and other sugars into the cell.) Once attached to ManYZ, SNIPE sits and waits for an invading phage. Some phages, including lambda, actually infect cells by pushing their DNA through this ManYZ channel. Lambda uses its "tail" to reach inside the protein channel, basically, and inject its DNA. When this physical touch happens, though, SNIPE is waiting. As soon as the phage DNA starts entering the cell, and passes through ManYZ and SNIPE, it gets immediately destroyed. This means that SNIPE is the first phage defense system discovered, so far, that uses spatial positioning at the injection site to destroy invaders. But there are caveats, of course. If you untether SNIPE from ManYZ, such that it can freely diffuse through the cell, it will chew up the bacterium's genome. It is not a highly discerning nuclease! Also, SNIPE is not found in most bacteria. A prior pangenome study, which sequenced lots of different microbes, found that roughly a third of well-studied bacterial lineages had at least one member with a SNIPE-like protein. (For this paper, they just ported one of those homologs into an E. coli laboratory strain.) And finally, because SNIPE's mechanism is tightly tied to ManYZ, it cannot be used to defend against phages that enter the cell through different routes. T4 phages, for example, inject their DNA straight through the cell membrane and into the cytoplasm, without interacting with ManYZ. This is a nice basic science paper. Applications TBD. (Just remember that scientists figured out that bacteria had a phage defense system, called CRISPR-Cas, many years before it was repurposed into a gene-editing tool.) P.S. The video below shows how cells with the SNIPE gene (middle row) kill invading phages, and thus continue growing and dividing. Empty vector (top row) refers to bacteria carrying a plasmid with no SNIPE gene; this is a control group. And SNIPE E414A refers to cells which received a mutated SNIPE gene, where the glutamate at position 414 has been changed to an alanine, thus destroying the protein's nuclease activity. These cells also die when they get infected with a phage.

Some microbes carry a protein, called SNIPE, that "chops up" phage DNA as it's being injected into the cell. This is a new mechanism for phage defense! CRISPR–Cas and restriction enzymes also evolved to fight against phages, but they work by recognizing sequences. SNIPE works, instead, by sensing "touch." SNIPE is a protein with about 500 amino acids. After it's made by the ribosome, it latches onto ManYZ, two proteins which sit on the cell's inner membrane. (ManYZ is an importer; it brings mannose and other sugars into the cell.) Once attached to ManYZ, SNIPE sits and waits for an invading phage. Some phages, including lambda, actually infect cells by pushing their DNA through this ManYZ channel. Lambda uses its "tail" to reach inside the protein channel, basically, and inject its DNA. When this physical touch happens, though, SNIPE is waiting. As soon as the phage DNA starts entering the cell, and passes through ManYZ and SNIPE, it gets immediately destroyed. This means that SNIPE is the first phage defense system discovered, so far, that uses spatial positioning at the injection site to destroy invaders. But there are caveats, of course. If you untether SNIPE from ManYZ, such that it can freely diffuse through the cell, it will chew up the bacterium's genome. It is not a highly discerning nuclease! Also, SNIPE is not found in most bacteria. A prior pangenome study, which sequenced lots of different microbes, found that roughly a third of well-studied bacterial lineages had at least one member with a SNIPE-like protein. (For this paper, they just ported one of those homologs into an E. coli laboratory strain.) And finally, because SNIPE's mechanism is tightly tied to ManYZ, it cannot be used to defend against phages that enter the cell through different routes. T4 phages, for example, inject their DNA straight through the cell membrane and into the cytoplasm, without interacting with ManYZ. This is a nice basic science paper. Applications TBD. (Just remember that scientists figured out that bacteria had a phage defense system, called CRISPR-Cas, many years before it was repurposed into a gene-editing tool.) P.S. The video below shows how cells with the SNIPE gene (middle row) kill invading phages, and thus continue growing and dividing. Empty vector (top row) refers to bacteria carrying a plasmid with no SNIPE gene; this is a control group. And SNIPE E414A refers to cells which received a mutated SNIPE gene, where the glutamate at position 414 has been changed to an alanine, thus destroying the protein's nuclease activity. These cells also die when they get infected with a phage.

Niko McCarty.

20,321 次观看 • 4 个月前

Tomorrow is a big day for me. I’m going to do an activity in my daughter’s class (she’s 5), and I’ll be teaching them what AI is. She’s excited about it, but honestly, I think I’m even more excited. I’ll tell them a little bit of history, we’ll play some games… I’ll try to make it as fun as possible, but always with a focus on ethical and responsible use. Of course, it won’t be a tutorial on how to use any tools, lol. They’re still very young, and in my opinion, it’ll be years before they actually need to start using these tools. But I think it’s important for them to start getting some awareness so they can be prepared for the future that’s coming their way.

Tomorrow is a big day for me. I’m going to do an activity in my daughter’s class (she’s 5), and I’ll be teaching them what AI is. She’s excited about it, but honestly, I think I’m even more excited. I’ll tell them a little bit of history, we’ll play some games… I’ll try to make it as fun as possible, but always with a focus on ethical and responsible use. Of course, it won’t be a tutorial on how to use any tools, lol. They’re still very young, and in my opinion, it’ll be years before they actually need to start using these tools. But I think it’s important for them to start getting some awareness so they can be prepared for the future that’s coming their way.

TechHalla

18,625 次观看 • 1 年前

Good Morning all **BACK IN STOCK** And from just £7.50 each Order yours here••••••> I have some exciting news!! I have designed and had made my very own Enamel pins… ♥️ The first 3 batches sold out pretty quickly so a massive thank you to all that snapped them up. Please feel free to tag me on here showing your new pins. I’ve just taken delivery of the fourth batch and I have 200 waiting for there new homes. These are perfectly priced for gifts or to keep yourself or even one for you and one to gift 😊🥳 All pins come with a backing card with every single one individually signed. Thank You 😊 x

Good Morning all BACK IN STOCK And from just £7.50 each Order yours here••••••> I have some exciting news!! I have designed and had made my very own Enamel pins… ♥️ The first 3 batches sold out pretty quickly so a massive thank you to all that snapped them up. Please feel free to tag me on here showing your new pins. I’ve just taken delivery of the fourth batch and I have 200 waiting for there new homes. These are perfectly priced for gifts or to keep yourself or even one for you and one to gift 😊🥳 All pins come with a backing card with every single one individually signed. Thank You 😊 x

Garry Floyd-Artist

267,896 次观看 • 1 年前

Last year during UNILAG’s Hall week, Go crzy announced that he would be pulling up to give out free merch on campus on 26th July 2025, the night before when he announced it, i took it open myself to push it heavily because this was a big deal to me and my guys, the night before which was the 25th of july, two of my insanely talented friends (Ledum and M!) spent the whole night working on paintings for go crzy to show him how much they appreciate his impact in the fashion and art industry, considering the fact that they are creatives as well. Fast forward to the next day, due to some issues go crzy wasn’t able to come in to UNILAG and my guys Leduum and M? have tried to reach out to GOCRAZY to give him the paintings since then but to no avail, today Go crzy is coming to UNILAG again and i really want to give him these paintings, it’s long overdue. Help me tag him to this post till he sees it GOCRAZY

Last year during UNILAG’s Hall week, Go crzy announced that he would be pulling up to give out free merch on campus on 26th July 2025, the night before when he announced it, i took it open myself to push it heavily because this was a big deal to me and my guys, the night before which was the 25th of july, two of my insanely talented friends (Ledum and M!) spent the whole night working on paintings for go crzy to show him how much they appreciate his impact in the fashion and art industry, considering the fact that they are creatives as well. Fast forward to the next day, due to some issues go crzy wasn’t able to come in to UNILAG and my guys Leduum and M? have tried to reach out to GOCRAZY to give him the paintings since then but to no avail, today Go crzy is coming to UNILAG again and i really want to give him these paintings, it’s long overdue. Help me tag him to this post till he sees it GOCRAZY

Lasisi 🇬🇧

32,729 次观看 • 3 个月前

🎙️| Enzo Fernández on his future amid links away from Chelsea🔵 “I love Chelsea, I really do. The fans have always supported me and I will always be grateful for that. But as a footballer, I need a team where they are ready to compete and win trophies, not Chelsea. you have to be honest with yourself. If a better opportunity comes along, you have to consider it. When I joined Chelsea, I believed the project would be competing for the biggest trophies every season. Instead, it feels like we’ve been stuck in the same cycle for years. Every season starts with promises, and every season ends with people talking about the future instead of celebrating success. I cannot spend my career waiting for things to happen. I am at a stage where I want to compete, I want to win, and I want to be fighting for the biggest trophies in football every year. Chelsea will always be a big club, but right now I need to think about my ambitions as well. If a club comes that is ready to win now, not in three or four years, then of course I have to listen. Football careers are short. You cannot keep asking players to be patient forever.”

🎙️| Enzo Fernández on his future amid links away from Chelsea🔵 “I love Chelsea, I really do. The fans have always supported me and I will always be grateful for that. But as a footballer, I need a team where they are ready to compete and win trophies, not Chelsea. you have to be honest with yourself. If a better opportunity comes along, you have to consider it. When I joined Chelsea, I believed the project would be competing for the biggest trophies every season. Instead, it feels like we’ve been stuck in the same cycle for years. Every season starts with promises, and every season ends with people talking about the future instead of celebrating success. I cannot spend my career waiting for things to happen. I am at a stage where I want to compete, I want to win, and I want to be fighting for the biggest trophies in football every year. Chelsea will always be a big club, but right now I need to think about my ambitions as well. If a club comes that is ready to win now, not in three or four years, then of course I have to listen. Football careers are short. You cannot keep asking players to be patient forever.”

Futy chels

82,423 次观看 • 26 天前