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New Raid Quest "vs Cell Max" is now underway in #DBXV2 ! Cell Max has appeared as a raid boss! Beware of high power and wide range of attacks, and let's all work together to face them! From March 23th (Thu) 8PM to 29th (Wed) 10PM PST/CET Learn more here:

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Dragon Ball Games

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118,888 Aufrufe • vor 3 Jahren •via X (Twitter)

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The most detailed 3D reconstruction of a cell ever created. Blows my mind every time. But what exactly are we looking at here? The average human cell contains: ~ 15-20 total distinct organelle types, totalling between ~1-10 million working together per cell. All these nano-machines in the cell are made up of proteins. ~ 8,000-10,000 distinct types of unique proteins, adding up to between 40 million - 10 trillion total proteins making up all those cellular systems. ~ 10,000 - 15,000 distinct types of RNA shuttling information around the cell, totalling up to ~10 million RNA molecules moving around the cell simultaneously. ~ Billions of Lipid molecules packed together into the cell membrane, which is also packed tightly with millions more protein-based nano-machines. And let's not forget billions of lines of DNA information to build and run it all. That's TRILLIONS of of individual molecular pieces working together to make a single cell function. That means there is more complexity in a single cell than humanity's largest cities. And people still believe this wasn't Divinely Designed. This is God's Glory on Display. But to make the point. A cell couldn't have evolved from some nebulous simpler "protocell" because even the simplest cells still require massive complexity. The "simplest" cell ever created was engineered by scientists knocking out pieces of a functional cell until it stopped functioning. Here is what they found is the absolute necessary minimal requirements of a cell to function: - Over ~531,000 lines of coded DNA information - 473 total genes to create hundreds of unique protein products (they later added 19 genes back in because the cell was so weak) - Hundreds of thousands of total proteins all working together - Extensive regulatory networks guiding all these interactions If the cell doesn't have all these systems in place, from the start... it doesn't live. Cell rely on an intricate network of complex systems, which are themselves built from complex interconnected pieces woven together into an incomprehensibly complex web of functionilty. Only intelligence has ever been observed creation vast interconnected systems like this. Life was clearly Created. It couldn't happen any other way.

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A single E. coli cell, placed on a dish, will become 70 billion cells in just 12 hours. That’s exponential growth. But a new preprint shows that it's possible to engineer E. coli to grow linearly instead, where only one daughter cell continues dividing and the other stops. First, some context. In nature, there is a bacterium called Mycobacterium smegmatis (initially discovered in 1884 in ulcers scraped from syphilis patients.) M. smegmatis is weird because it divides asymmetrically. These cells grow only from one end, and all their cell wall biosynthesis machinery is located on that one end. So when the cell divides, one daughter gets this machinery and the other gets nothing. The daughter that gets the machinery can keep dividing immediately, but the other daughter has to remake all that machinery from scratch, so its growth is delayed. E. coli doesn’t grow like this. When it divides, it pinches in the middle and splits everything evenly. Enzymes, metabolites, and proteins get partitioned more or less randomly between the two daughters. For the new preprint, though, researchers engineered E. coli to behave more like M. smegmatis. Here is how they did it: First, they deleted a gene called cyaA, which encodes an enzyme (adenylate cyclase) that makes a molecule called cAMP. cAMP is SUPER IMPORTANT! It is a nutrient sensor that instructs E. coli to switch on genes that help it digest non-glucose carbon sources when glucose is scarce. Without cAMP, E. coli cells growing on alternative carbon sources will starve; they won’t know how to eat the food. Next, they added back a “split” version of the cyaA gene into the cells. In other words, they split the gene in two so that each half of the enzyme is made separately. Cells can only make cAMP, and thus eat non-glucose carbon sources, if these two halves come together. To facilitate that “coming together,” the researchers also fused the split cyaA proteins to sticky proteins that clump together, and to a fluorescent protein (to make it easy to track these molecules in the cell.) So now some interesting things start to happen if you grow E. coli on a growth medium lacking glucose. As the cell grows, its cyaA “halves” start clumping together into a giant ball. Inside the aggregate, the two enzyme halves come together and make cAMP. And when the cell gets big enough and divides, the clump of cyaA RANDOMLY goes to either daughter cell #1 or #2. The daughter that gets the aggregate (called PA+ in this paper) can keep dividing. The daughter that doesn’t (PA–) cannot. It still grows a few times — about four divisions — because it inherits some leftover cAMP from its mother. But after that, the metabolite is diluted away, and the cell stops growing. PA+ cells went through about 23 divisions on average before their aggregate decayed. And the population of cells, as a whole, grew linearly. This paper is cool because there are many applications where exponential growth is too unpredictable and, perhaps, unsafe. If you want to engineer bacteria to deliver drugs, clean up waste, or live in the gut, you don’t want them to double uncontrollably. This paper shows you can make them expand in a controlled, linear way. Alas, mutations could break this whole engineered system. A mutation that restores cyaA, for example, would give cells a new way to make cAMP. Mutations that make the aggregates split between daughters would break the asymmetry, too. But still, I really enjoy proof-of-concept engineering papers like this.

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WOW! This is the view from the The George Washington bridge running from Manhattan, New York to New Jersey. - This is all stemming from Central Canada, where wildfires are burning in the provinces of Ontario and Quebec. - In total there are 211 reported wildfires burning in these two provinces, with 145 of them considered "out of control". - These fires started because of unseasonable hot and dry conditions, and have already forced 10,000 residents to evacuate their homes in Quebec. I think what this shows us is how fragile and interconnected the entire world really is environmentally. An event that takes place in another country can have major impacts and implications on our own. We are all in this thing together. Let's get along and work together to figure things out. The environment is a great place to start.

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Look at Shujaa making herself quite at home in Ithumba! Fresh graduates Weka and Shujaa have recently made the big move from Nairobi Nursery to our Ithumba Reintegration Unit, and it's wonderful to see how quickly they're adapting to their new surroundings. Shujaa has discovered the joy of rock climbing - a perfect way to build strength and confidence in her new environment! The transition from our Nursery to a Reintegration Unit is a major milestone for any orphan. Here at Ithumba, the duo will learn essential skills from older orphans, ex-orphans, and wild elephants, gradually becoming more independent, all under the watchful care of their dedicated Keepers. From Nursery babies to Ithumba adventurers - every step takes them closer to their wild future! Support Shujaa’s journey:

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I'm sure you've all noticed the 'AI is slowing down' news stories every few weeks for multiple years now - so I've pulled a tracker together to see who and when wrote these stories. There is quite a range, some are just outright wrong, others point to a reasonable limitation at the time but missing the bigger arc of progress. All of these stories were appearing as we were getting reasoning models, open source models, increasing competition from more players and skyrocketing revenue for the labs. Link to the tracker in the comments

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