Video yükleniyor...

Video Yüklenemedi

Ana Sayfaya Dön

A cell dividing into two daughter cells videoed through a microscope. Chromosomes are labeled in pink. Technique: differential interference microscopy (DIC) and fluorescence. #CellBiology

MAG2ART's profile picture

Dylan Burnette

33,447 subscribers

79,078 görüntüleme • 6 ay önce •via X (Twitter)

Sağlık & İyilikBilim & Teknoloji#CellBiology
LIVE

Anya Rossi• Live Now

Private livecam show

0 Yorum

Yorum bulunmuyor

Orijinal gönderinin yorumları burada görünecek

Benzer Videolar

A cell videoed through a microscope. DNA in the nucleus (green) and mitochondria (purple) are shown. #CellBiology
0:10

A cell videoed through a microscope. DNA in the nucleus (green) and mitochondria (purple) are shown. #CellBiology

Dylan Burnette

52,603 görüntüleme • 2 yıl önce

A cell videoed through a microscope. DNA (cyan), mitochondria (yellow), and VASP (magenta) are shown. #CellBiology
0:16

A cell videoed through a microscope. DNA (cyan), mitochondria (yellow), and VASP (magenta) are shown. #CellBiology

Dylan Burnette

119,340 görüntüleme • 3 yıl önce

A cell videoed through a microscope. DNA in the nucleus (green) and the powerhouses/overlords of the cell, mitochondria (purple), are shown. #CellBiology
0:10

A cell videoed through a microscope. DNA in the nucleus (green) and the powerhouses/overlords of the cell, mitochondria (purple), are shown. #CellBiology

Dylan Burnette

100,600 görüntüleme • 1 yıl önce

A melanoma cancer cell videoed through a microscope. DNA in the nucleus (yellow), mitochondria (cyan), and VASP (magenta) are shown. #CellBiology
0:20

A melanoma cancer cell videoed through a microscope. DNA in the nucleus (yellow), mitochondria (cyan), and VASP (magenta) are shown. #CellBiology

Dylan Burnette

23,484 görüntüleme • 2 yıl önce

A Golgi apparatus protein in a cell videoed through a microscope. The Golgi is saturated to show the vesicles trafficking around the cell. #CellBiology
0:10

A Golgi apparatus protein in a cell videoed through a microscope. The Golgi is saturated to show the vesicles trafficking around the cell. #CellBiology

Dylan Burnette

60,887 görüntüleme • 3 yıl önce

A cancer cell videoed through a microscope. It has 3 nuclei (magenta). The powerhouse/overlords of the cell, mitochondria, are also shown (green). #CellBiology
0:10

A cancer cell videoed through a microscope. It has 3 nuclei (magenta). The powerhouse/overlords of the cell, mitochondria, are also shown (green). #CellBiology

Dylan Burnette

16,967 görüntüleme • 4 ay önce

Mitosis is a part of the cell cycle in which replicated chromosomes are separated into two new nuclei. Such a division gives rise to genetically identical cells in which the total number of chromosomes is maintained [source:
0:17

Mitosis is a part of the cell cycle in which replicated chromosomes are separated into two new nuclei. Such a division gives rise to genetically identical cells in which the total number of chromosomes is maintained [source:

Massimo

505,583 görüntüleme • 3 yıl önce

#FluorescenceFriday #cellbiology #bioart The actin cytoskeleton at 60 nm resolution and 3 frames/min, and its association with early endosomes (Rab5a, green), as seen in a COS-7 cell by photoactivated nonlinear structured illumination microscopy
0:13

#FluorescenceFriday #cellbiology #bioart The actin cytoskeleton at 60 nm resolution and 3 frames/min, and its association with early endosomes (Rab5a, green), as seen in a COS-7 cell by photoactivated nonlinear structured illumination microscopy

Eric Betzig

25,853 görüntüleme • 2 yıl önce

We present oblique plane microscopy with adaptive optics (AO): One deformable mirror corrects both the light-sheet and the fluorescence detection. Movie shows cancer cells in a zebrafish xenograft model, before and after application of AO.
0:14

We present oblique plane microscopy with adaptive optics (AO): One deformable mirror corrects both the light-sheet and the fluorescence detection. Movie shows cancer cells in a zebrafish xenograft model, before and after application of AO.

Reto Fiolka

13,452 görüntüleme • 2 yıl önce

Why not show real data instead of a 2D cartoon? Left diagonal: computationally separated 3D volume-rendered movies at 15 sec intervals for 19 minutes of chromosomes, mitochondria, and the ER in the same field of dividing pig kidney cells. Middle diagonal: overlapped color channels computationally sliced in slabs through the volume to reveal internal structure and interactions throughout mitosis. Upper right: zoomed in view in one slab, showing organelle rearrangements in the left cell from metaphase through telophase.
0:15

Why not show real data instead of a 2D cartoon? Left diagonal: computationally separated 3D volume-rendered movies at 15 sec intervals for 19 minutes of chromosomes, mitochondria, and the ER in the same field of dividing pig kidney cells. Middle diagonal: overlapped color channels computationally sliced in slabs through the volume to reveal internal structure and interactions throughout mitosis. Upper right: zoomed in view in one slab, showing organelle rearrangements in the left cell from metaphase through telophase.

Eric Betzig

19,923 görüntüleme • 3 ay önce

The head of a bearded dragon embryo (50 days old), imaged with light sheet fluorescence microscopy 🦎🔬
0:15

The head of a bearded dragon embryo (50 days old), imaged with light sheet fluorescence microscopy 🦎🔬

Rory Cooper

19,410 görüntüleme • 1 yıl önce

A white blood cell navigates through red blood cells, scanning for threats
0:13

A white blood cell navigates through red blood cells, scanning for threats

HOW THINGS WORK

52,359 görüntüleme • 11 ay önce

Nice example of actin retrograde flow in a gigantic lamellipodium. #cytoskeleton, #actin, #microscopy, #cell-motility, #science
0:10

Nice example of actin retrograde flow in a gigantic lamellipodium. #cytoskeleton, #actin, #microscopy, #cell-motility, #science

Jeffrey van Haren

20,435 görüntüleme • 2 yıl önce

A single E. coli cell, placed on a dish, will become 70 billion cells in just 12 hours. That’s exponential growth. But a new preprint shows that it's possible to engineer E. coli to grow linearly instead, where only one daughter cell continues dividing and the other stops. First, some context. In nature, there is a bacterium called Mycobacterium smegmatis (initially discovered in 1884 in ulcers scraped from syphilis patients.) M. smegmatis is weird because it divides asymmetrically. These cells grow only from one end, and all their cell wall biosynthesis machinery is located on that one end. So when the cell divides, one daughter gets this machinery and the other gets nothing. The daughter that gets the machinery can keep dividing immediately, but the other daughter has to remake all that machinery from scratch, so its growth is delayed. E. coli doesn’t grow like this. When it divides, it pinches in the middle and splits everything evenly. Enzymes, metabolites, and proteins get partitioned more or less randomly between the two daughters. For the new preprint, though, researchers engineered E. coli to behave more like M. smegmatis. Here is how they did it: First, they deleted a gene called cyaA, which encodes an enzyme (adenylate cyclase) that makes a molecule called cAMP. cAMP is SUPER IMPORTANT! It is a nutrient sensor that instructs E. coli to switch on genes that help it digest non-glucose carbon sources when glucose is scarce. Without cAMP, E. coli cells growing on alternative carbon sources will starve; they won’t know how to eat the food. Next, they added back a “split” version of the cyaA gene into the cells. In other words, they split the gene in two so that each half of the enzyme is made separately. Cells can only make cAMP, and thus eat non-glucose carbon sources, if these two halves come together. To facilitate that “coming together,” the researchers also fused the split cyaA proteins to sticky proteins that clump together, and to a fluorescent protein (to make it easy to track these molecules in the cell.) So now some interesting things start to happen if you grow E. coli on a growth medium lacking glucose. As the cell grows, its cyaA “halves” start clumping together into a giant ball. Inside the aggregate, the two enzyme halves come together and make cAMP. And when the cell gets big enough and divides, the clump of cyaA RANDOMLY goes to either daughter cell #1 or #2. The daughter that gets the aggregate (called PA+ in this paper) can keep dividing. The daughter that doesn’t (PA–) cannot. It still grows a few times — about four divisions — because it inherits some leftover cAMP from its mother. But after that, the metabolite is diluted away, and the cell stops growing. PA+ cells went through about 23 divisions on average before their aggregate decayed. And the population of cells, as a whole, grew linearly. This paper is cool because there are many applications where exponential growth is too unpredictable and, perhaps, unsafe. If you want to engineer bacteria to deliver drugs, clean up waste, or live in the gut, you don’t want them to double uncontrollably. This paper shows you can make them expand in a controlled, linear way. Alas, mutations could break this whole engineered system. A mutation that restores cyaA, for example, would give cells a new way to make cAMP. Mutations that make the aggregates split between daughters would break the asymmetry, too. But still, I really enjoy proof-of-concept engineering papers like this.
0:14

A single E. coli cell, placed on a dish, will become 70 billion cells in just 12 hours. That’s exponential growth. But a new preprint shows that it's possible to engineer E. coli to grow linearly instead, where only one daughter cell continues dividing and the other stops. First, some context. In nature, there is a bacterium called Mycobacterium smegmatis (initially discovered in 1884 in ulcers scraped from syphilis patients.) M. smegmatis is weird because it divides asymmetrically. These cells grow only from one end, and all their cell wall biosynthesis machinery is located on that one end. So when the cell divides, one daughter gets this machinery and the other gets nothing. The daughter that gets the machinery can keep dividing immediately, but the other daughter has to remake all that machinery from scratch, so its growth is delayed. E. coli doesn’t grow like this. When it divides, it pinches in the middle and splits everything evenly. Enzymes, metabolites, and proteins get partitioned more or less randomly between the two daughters. For the new preprint, though, researchers engineered E. coli to behave more like M. smegmatis. Here is how they did it: First, they deleted a gene called cyaA, which encodes an enzyme (adenylate cyclase) that makes a molecule called cAMP. cAMP is SUPER IMPORTANT! It is a nutrient sensor that instructs E. coli to switch on genes that help it digest non-glucose carbon sources when glucose is scarce. Without cAMP, E. coli cells growing on alternative carbon sources will starve; they won’t know how to eat the food. Next, they added back a “split” version of the cyaA gene into the cells. In other words, they split the gene in two so that each half of the enzyme is made separately. Cells can only make cAMP, and thus eat non-glucose carbon sources, if these two halves come together. To facilitate that “coming together,” the researchers also fused the split cyaA proteins to sticky proteins that clump together, and to a fluorescent protein (to make it easy to track these molecules in the cell.) So now some interesting things start to happen if you grow E. coli on a growth medium lacking glucose. As the cell grows, its cyaA “halves” start clumping together into a giant ball. Inside the aggregate, the two enzyme halves come together and make cAMP. And when the cell gets big enough and divides, the clump of cyaA RANDOMLY goes to either daughter cell #1 or #2. The daughter that gets the aggregate (called PA+ in this paper) can keep dividing. The daughter that doesn’t (PA–) cannot. It still grows a few times — about four divisions — because it inherits some leftover cAMP from its mother. But after that, the metabolite is diluted away, and the cell stops growing. PA+ cells went through about 23 divisions on average before their aggregate decayed. And the population of cells, as a whole, grew linearly. This paper is cool because there are many applications where exponential growth is too unpredictable and, perhaps, unsafe. If you want to engineer bacteria to deliver drugs, clean up waste, or live in the gut, you don’t want them to double uncontrollably. This paper shows you can make them expand in a controlled, linear way. Alas, mutations could break this whole engineered system. A mutation that restores cyaA, for example, would give cells a new way to make cAMP. Mutations that make the aggregates split between daughters would break the asymmetry, too. But still, I really enjoy proof-of-concept engineering papers like this.

Niko McCarty.

57,988 görüntüleme • 10 ay önce

Congrats Andrew Weems and everyone involved on elucidating how blebs help cancer cells avoid anoikis (cell death when losing attachment). Study involves 3D microscopy, and doing math on cell surfaces, courtesy of the @DanuserLab computer vision wizards.
0:14

Congrats Andrew Weems and everyone involved on elucidating how blebs help cancer cells avoid anoikis (cell death when losing attachment). Study involves 3D microscopy, and doing math on cell surfaces, courtesy of the @DanuserLab computer vision wizards.

Reto Fiolka

10,354 görüntüleme • 3 yıl önce

Mitochondria in a cell. What is going on? Make a guess. Test the guess. Find joy in being wrong. Guess again......... #Science #CellBiology #FindJoyInBeingWrong
0:10

Mitochondria in a cell. What is going on? Make a guess. Test the guess. Find joy in being wrong. Guess again......... #Science #CellBiology #FindJoyInBeingWrong

Dylan Burnette

63,438 görüntüleme • 3 yıl önce

#cellbiology #bioart The actin #cytoskeleton (green) is linked to a variety of transmembrane proteins through alpha-actinin (magenta), with such linkages being particularly dense in membrane ruffles and filopodia. As seen at 3 frames/min for 8 min by linear and non-linear structured illumination microscopy (97 and 60 nm resolution, respectively).
0:16

#cellbiology #bioart The actin #cytoskeleton (green) is linked to a variety of transmembrane proteins through alpha-actinin (magenta), with such linkages being particularly dense in membrane ruffles and filopodia. As seen at 3 frames/min for 8 min by linear and non-linear structured illumination microscopy (97 and 60 nm resolution, respectively).

Eric Betzig

25,403 görüntüleme • 1 yıl önce

What does the endoplasmic reticulum look like in a live cell, thanks to super-resolution microscopy? Nature Methods
0:13

What does the endoplasmic reticulum look like in a live cell, thanks to super-resolution microscopy? Nature Methods

Eric Topol

24,010 görüntüleme • 2 ay önce

Check out this bearded dragon embryo (~30 days old) imaged with light sheet fluorescence microscopy 🦎🔬
0:13

Check out this bearded dragon embryo (~30 days old) imaged with light sheet fluorescence microscopy 🦎🔬

Rory Cooper

38,922 görüntüleme • 1 yıl önce

Bacteria glowing with green fluorescence move inside a fungal cell. In this video, the bacteria seem like an infection, but in successive generations, the two organisms will adapt to each other until they find endosymbiotic balance.
0:20

Bacteria glowing with green fluorescence move inside a fungal cell. In this video, the bacteria seem like an infection, but in successive generations, the two organisms will adapt to each other until they find endosymbiotic balance.

Quanta Magazine

18,609 görüntüleme • 7 ay önce