Video wird geladen...

Video konnte nicht geladen werden

Zur Startseite

We very much welcome a postdoc who is interested in single-nucleosome imaging/analysis to reveal how chromatin behaves in living human cells! NIG offers Postdoctoral Fellowship:

kazu_maeshima's profile picture

Kazuhiro Maeshima

2,076 subscribers

86,244 Aufrufe • vor 3 Jahren •via X (Twitter)

LIVE

Anya Rossi• Live Now

Private livecam show

10 Kommentare

Profilbild von Kazuhiro Maeshima
Kazuhiro Maeshimavor 3 Jahren

Left video, oblique illumination or HILO (image series from epi to TIR) with @nikonsolutions TIRF unit. Right, single-nucleosome video with @hama_photon_pr sCMOS camera. Each dot represents a single nucleosome. They are swaying in living human cells!

Profilbild von Aakeel Ahmad
Aakeel Ahmadvor 3 Jahren

Looks exciting!

Profilbild von Kazuhiro Maeshima
Kazuhiro Maeshimavor 3 Jahren

Thank you!

Profilbild von Evangelos Gatzogiannis
Evangelos Gatzogiannisvor 3 Jahren

Oblique (HiLo) microscopy is the tool for this kind of imaging. Nice side-by-side

Profilbild von Kazuhiro Maeshima
Kazuhiro Maeshimavor 3 Jahren

Thank you! Indeed, oblique (HILO) illumination is a great way to see the movements of molecules in living cells.

Profilbild von Alex hego
Alex hegovor 3 Jahren

@Nicolas26538817 What do you think about lattice lightsheet? 🤔

Profilbild von Kazuhiro Maeshima
Kazuhiro Maeshimavor 3 Jahren

@ogehxela @Nicolas26538817 The lattice light sheet is more sophisticated. Oblique illumination, or HILO, is convenient for getting light-sheet illumination.

Profilbild von Victor Leyton
Victor Leytonvor 3 Jahren

Any interest in applying this amazing technology to study NLS nuclear transport???

Profilbild von Kazuhiro Maeshima
Kazuhiro Maeshimavor 3 Jahren

Great point! You can see how the molecules with NLS bind to NPCs and go through them. Originally oblique illumination or HILO was invented for such a study. Please see:

Profilbild von Dan
Danvor 3 Jahren

@DrBrianStrahl

Ähnliche Videos

Live-cell chromatin imaging is no longer a question of HOW. It is a question of WHY: why dynamics is crucial for cells. Yanyu Zhu (@yanyu_zhu), a brilliant postdoc, leads our new Nature Reviews Genetics review covering a decade of this field to answer the question
0:24

Live-cell chromatin imaging is no longer a question of HOW. It is a question of WHY: why dynamics is crucial for cells. Yanyu Zhu (@yanyu_zhu), a brilliant postdoc, leads our new Nature Reviews Genetics review covering a decade of this field to answer the question

Stanley Qi

18,791 Aufrufe • vor 2 Monaten

Our linker histone H1 paper is out Science Advances: Masa A. Shimazoe et al. reveal that H1 acts as a liquid-like "glue" to organize chromatin in living cells. 🎉 Fantastic collab with Rosana Collepardo, Jan Huertas, Charlie Phillips and others—huge thanks! 🙌 ½
0:30

Our linker histone H1 paper is out Science Advances: Masa A. Shimazoe et al. reveal that H1 acts as a liquid-like "glue" to organize chromatin in living cells. 🎉 Fantastic collab with Rosana Collepardo, Jan Huertas, Charlie Phillips and others—huge thanks! 🙌 ½

Kazuhiro Maeshima

35,577 Aufrufe • vor 2 Monaten

Our new preprint is out＠bioRxiv: Masa A. Shimazoe et al. reveal that linker histone H1 acts as a liquid-like "glue" to organize chromatin in living cells. 🎉 Fantastic collab with Rosana Collepardo, Charlie Phillips, Jan Huertas and others—huge thanks! 🙌 1/2
0:30

Our new preprint is out＠bioRxiv: Masa A. Shimazoe et al. reveal that linker histone H1 acts as a liquid-like "glue" to organize chromatin in living cells. 🎉 Fantastic collab with Rosana Collepardo, Charlie Phillips, Jan Huertas and others—huge thanks! 🙌 1/2

Kazuhiro Maeshima

15,611 Aufrufe • vor 1 Jahr

"How much we spend a month living in Miami"
1:01

"How much we spend a month living in Miami"

Financial Dystopia

2,405,650 Aufrufe • vor 10 Monaten

The Marlins are "very much interested" in Pete Fairbanks, according to Isaac Azout. "They've even upped their offers to the mid-teens for a one-year deal."
1:43

The Marlins are "very much interested" in Pete Fairbanks, according to Isaac Azout. "They've even upped their offers to the mid-teens for a one-year deal."

Foul Territory

39,371 Aufrufe • vor 6 Monaten

HiP-CT is a revolutionary imaging technique that reveals the human body in extraordinary detail. Zoom from an entire organ down to cells and blood vessels. Here, exploring the human brain at micron-scale. [📹 Paul Tafforeau]
0:31

HiP-CT is a revolutionary imaging technique that reveals the human body in extraordinary detail. Zoom from an entire organ down to cells and blood vessels. Here, exploring the human brain at micron-scale. [📹 Paul Tafforeau]

Massimo

80,176 Aufrufe • vor 6 Monaten

Our latest📰! Out today in Molecular Cell We used single-molecule imaging to directly observe human BRCA2 tumour suppressor in action! Spearheaded by Ondrej Belan & Luke Greenhough. A thread. (1/10)
0:24

Our latest📰! Out today in Molecular Cell We used single-molecule imaging to directly observe human BRCA2 tumour suppressor in action! Spearheaded by Ondrej Belan & Luke Greenhough. A thread. (1/10)

The Boulton Lab

48,172 Aufrufe • vor 2 Jahren

Our new paper about the structural mechanism by which RAD51 assembles at double-stranded DNA break sites on chromatin has been published in Nature! #CryoEM #Nucleosome #RAD51 #DNArepair
0:47

Our new paper about the structural mechanism by which RAD51 assembles at double-stranded DNA break sites on chromatin has been published in Nature! #CryoEM #Nucleosome #RAD51 #DNArepair

Kurumizaka Lab

72,776 Aufrufe • vor 2 Jahren

Destiny's analysis of me is... interesting. I think we are just interested in different things and coming at this from 2 very different approaches.
7:25

Destiny's analysis of me is... interesting. I think we are just interested in different things and coming at this from 2 very different approaches.

Warren Smith

211,278 Aufrufe • vor 1 Jahr

Did you know that applying for the #MSCA Postdoctoral Fellowships call is not the only way to become a MSCA fellow? On the #Euraxess portal there are plenty of postdoc vacancies for you to do research in a MSCA-funded project! Hear from Miguel how the MSCA impacted his career👇
1:06

Did you know that applying for the #MSCA Postdoctoral Fellowships call is not the only way to become a MSCA fellow? On the #Euraxess portal there are plenty of postdoc vacancies for you to do research in a MSCA-funded project! Hear from Miguel how the MSCA impacted his career👇

Marie Skłodowska-Curie Actions

28,375 Aufrufe • vor 1 Jahr

Did you know that applying for the #MSCA Postdoctoral Fellowships call is not the only way to become a MSCA fellow? On the Euraxess portal there are plenty of postdoc vacancies for you to do research in a MSCA-funded project! Hear from Miguel how the MSCA impacted his career 👇
1:06

Did you know that applying for the #MSCA Postdoctoral Fellowships call is not the only way to become a MSCA fellow? On the Euraxess portal there are plenty of postdoc vacancies for you to do research in a MSCA-funded project! Hear from Miguel how the MSCA impacted his career 👇

Marie Skłodowska-Curie Actions

15,138 Aufrufe • vor 2 Jahren

"Good cinema is what we can believe & bad cinema is what we can't believe. What you see & believe in is very much what I'm interested in." --- Abbas Kiarostami
0:47

"Good cinema is what we can believe & bad cinema is what we can't believe. What you see & believe in is very much what I'm interested in." --- Abbas Kiarostami

DepressedBergman

166,416 Aufrufe • vor 2 Jahren

🧬A new imaging technique developed by the Liu Lab uses a novel DNA barcode system to track hundreds of RNA & protein molecules in single cells within thick biological samples, providing a full picture of how these structures are organized inside tissues.
0:33

🧬A new imaging technique developed by the Liu Lab uses a novel DNA barcode system to track hundreds of RNA & protein molecules in single cells within thick biological samples, providing a full picture of how these structures are organized inside tissues.

HHMI | Janelia

79,474 Aufrufe • vor 1 Jahr

How can a single cell learn without a brain? We explore this in my new paper with Wallace Marshall! We discovered that single cells may learn using molecules similar to those that animal brains use to learn, like CaMKII. Cells can also propagate memory states to their progeny! 🧵1/n
0:21

How can a single cell learn without a brain? We explore this in my new paper with Wallace Marshall! We discovered that single cells may learn using molecules similar to those that animal brains use to learn, like CaMKII. Cells can also propagate memory states to their progeny! 🧵1/n

Deepa Rajan, PhD

75,536 Aufrufe • vor 2 Monaten

1.Stem cells reside in specialized microenvironments that precisely regulate their behavior. For this reason they are best studied in their in vivo context. A new paper from our lab describes a method for long-term imaging & quantitative analysis of hematopoiesis in vivo in flies
2:08

1.Stem cells reside in specialized microenvironments that precisely regulate their behavior. For this reason they are best studied in their in vivo context. A new paper from our lab describes a method for long-term imaging & quantitative analysis of hematopoiesis in vivo in flies

Tanentzapf Lab

27,031 Aufrufe • vor 3 Jahren

Excited to share our latest work and first venture into the chromatin field. We visualized for the first time that shelterin factor TRF1 binds and modulates telomeric nucleosome. hongmiao @AutomneNine Great collaboration with the Robinson's group
1:08

Excited to share our latest work and first venture into the chromatin field. We visualized for the first time that shelterin factor TRF1 binds and modulates telomeric nucleosome. hongmiao @AutomneNine Great collaboration with the Robinson's group

Kelly Nguyen

21,702 Aufrufe • vor 2 Jahren

All cells make “sounds” healthy cells “sound” one ways and cancerous cells “sound”completely different. In the future we will be able to detect a single cancer cell, by its “sound”. Electronic medicine is the future.
1:23

All cells make “sounds” healthy cells “sound” one ways and cancerous cells “sound”completely different. In the future we will be able to detect a single cancer cell, by its “sound”. Electronic medicine is the future.

Brian Roemmele

188,882 Aufrufe • vor 2 Jahren

If anyone is interested in our advanced fellowship please send your CV to Karey.cerqua@ascension.org The interviews will be November 2nd. We pray for peace for all.
1:41

If anyone is interested in our advanced fellowship please send your CV to [email protected] The interviews will be November 2nd. We pray for peace for all.

Amir Kaki, MD

64,896 Aufrufe • vor 2 Jahren

In chapter 4 of our science explainer series, we continue our journey inside a human cell to demonstrate how the Prime Editor is intended to correct a single base mutation.
1:23

In chapter 4 of our science explainer series, we continue our journey inside a human cell to demonstrate how the Prime Editor is intended to correct a single base mutation.

Prime Medicine

43,965 Aufrufe • vor 3 Jahren

Does the government of Uganda have a regulation on how much land a single individual is supposed to own? Late was indeed very rich in terms of land ownership!!!
1:16

Does the government of Uganda have a regulation on how much land a single individual is supposed to own? Late was indeed very rich in terms of land ownership!!!

Cecile

19,580 Aufrufe • vor 1 Monat