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We very much welcome a postdoc who is interested in single-nucleosome imaging/analysis to reveal how chromatin behaves in living human cells! NIG offers Postdoctoral Fellowship:

Kazuhiro Maeshima

2,076 subscribers

86,244 views • 3 years ago •via X (Twitter)

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10 Comments

Kazuhiro Maeshima3 years ago

Left video, oblique illumination or HILO (image series from epi to TIR) with @nikonsolutions TIRF unit. Right, single-nucleosome video with @hama_photon_pr sCMOS camera. Each dot represents a single nucleosome. They are swaying in living human cells!

Aakeel Ahmad3 years ago

Looks exciting!

Kazuhiro Maeshima3 years ago

Thank you!

Evangelos Gatzogiannis3 years ago

Oblique (HiLo) microscopy is the tool for this kind of imaging. Nice side-by-side

Kazuhiro Maeshima3 years ago

Thank you! Indeed, oblique (HILO) illumination is a great way to see the movements of molecules in living cells.

Alex hego3 years ago

@Nicolas26538817 What do you think about lattice lightsheet? 🤔

Kazuhiro Maeshima3 years ago

@ogehxela @Nicolas26538817 The lattice light sheet is more sophisticated. Oblique illumination, or HILO, is convenient for getting light-sheet illumination.

Victor Leyton3 years ago

Any interest in applying this amazing technology to study NLS nuclear transport???

Kazuhiro Maeshima3 years ago

Great point! You can see how the molecules with NLS bind to NPCs and go through them. Originally oblique illumination or HILO was invented for such a study. Please see:

Dan3 years ago

@DrBrianStrahl

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