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We very much welcome a postdoc who is interested in single-nucleosome imaging/analysis to reveal how chromatin behaves in living human cells! NIG offers Postdoctoral Fellowship:

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Kazuhiro Maeshima

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86,244 görüntüleme • 3 yıl önce •via X (Twitter)

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Kazuhiro Maeshima3 yıl önce

Left video, oblique illumination or HILO (image series from epi to TIR) with @nikonsolutions TIRF unit. Right, single-nucleosome video with @hama_photon_pr sCMOS camera. Each dot represents a single nucleosome. They are swaying in living human cells!

Aakeel Ahmad profil fotoğrafı
Aakeel Ahmad3 yıl önce

Looks exciting!

Kazuhiro Maeshima profil fotoğrafı
Kazuhiro Maeshima3 yıl önce

Thank you!

Evangelos Gatzogiannis profil fotoğrafı
Evangelos Gatzogiannis3 yıl önce

Oblique (HiLo) microscopy is the tool for this kind of imaging. Nice side-by-side

Kazuhiro Maeshima profil fotoğrafı
Kazuhiro Maeshima3 yıl önce

Thank you! Indeed, oblique (HILO) illumination is a great way to see the movements of molecules in living cells.

Alex hego profil fotoğrafı
Alex hego3 yıl önce

@Nicolas26538817 What do you think about lattice lightsheet? 🤔

Kazuhiro Maeshima profil fotoğrafı
Kazuhiro Maeshima3 yıl önce

@ogehxela @Nicolas26538817 The lattice light sheet is more sophisticated. Oblique illumination, or HILO, is convenient for getting light-sheet illumination.

Victor Leyton profil fotoğrafı
Victor Leyton3 yıl önce

Any interest in applying this amazing technology to study NLS nuclear transport???

Kazuhiro Maeshima profil fotoğrafı
Kazuhiro Maeshima3 yıl önce

Great point! You can see how the molecules with NLS bind to NPCs and go through them. Originally oblique illumination or HILO was invented for such a study. Please see:

Dan profil fotoğrafı
Dan3 yıl önce

@DrBrianStrahl

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