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Another beautiful 3D analysis of mouse eye vasculature using tissue clearing and light-sheet microscopy. Unlike many others (but like EyeDISCO) it can be applied to pigmented eyes Luc Krimpenfort et al., Lars Jakobsson lab

Alain Chédotal

5,219 subscribers

97,022 Aufrufe • vor 3 Jahren •via X (Twitter)

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Anya Rossi• Live Now

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10 Kommentare

Profilbild von Guillaume Hoeffel

Guillaume Hoeffelvor 3 Jahren

@LucKrimpenfort Beautiful indeed!

Profilbild von edward lachica

edward lachicavor 3 Jahren

@LucKrimpenfort This is gorgeous. Eager to read the paper.

Profilbild von Barry Doyle

Barry Doylevor 3 Jahren

@LucKrimpenfort Check this out @HarrisonCaddy

Profilbild von Alek Erickson

Alek Ericksonvor 3 Jahren

@LucKrimpenfort Congratulations on this nice looking work @LucKrimpenfort

Profilbild von Elkhan Yusifov

Elkhan Yusifovvor 3 Jahren

@LucKrimpenfort Wow, wonderful!

Profilbild von Simon H Parson

Simon H Parsonvor 3 Jahren

@LucKrimpenfort Fantastic, I would love to try that on mouse #SMA retinas , where we have a dramatic phenotype

Profilbild von Phil Nickerson

Phil Nickersonvor 3 Jahren

@LucKrimpenfort Fantastic prep and imaging. You are incorrect, however. Gurdita et al., iScience achieved this in pigmented eyes. I look forward to reading your published protocol 👍🏻

Profilbild von Alain Chédotal

Alain Chédotalvor 3 Jahren

@LucKrimpenfort So did we 😉

Profilbild von Ryan T. Scott

Ryan T. Scottvor 3 Jahren

@LucKrimpenfort . @KeithSiew @diary_cc seems like content you both would appreciate ♥️

Profilbild von Caroline Menard

Caroline Menardvor 3 Jahren

@SiegenthalerLab @LucKrimpenfort 🤯🤯🤯

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57,895 Aufrufe • vor 6 Monaten

Castor oil is a beautiful healing agent that has been used for centuries to reduce inflammation, support circulation, and encourage detoxification. When applied to the skin, especially as a castor oil pack, it works gently and consistently to move the lymphatic system and soothe irritated tissues. But when it comes to cataracts, we must understand the anatomy. A cataract forms inside the lens, not on the surface of the eye. This means that while castor oil may hydrate the eyelids, calm irritation, and support the delicate tissues around the eye, it cannot penetrate into the lens to dissolve a cataract. Some people do use sterile, ophthalmic castor oil for dry eyes, and it may help with moisture and tear-film stability. But it has not been shown in human studies to reverse cataracts. So the remedy remains valuable — just in the right place. Castor oil can support healing, but we must always match the treatment to the tissue involved. Use it wisely, use it safely, and always protect the precious gift of sight.

Castor oil is a beautiful healing agent that has been used for centuries to reduce inflammation, support circulation, and encourage detoxification. When applied to the skin, especially as a castor oil pack, it works gently and consistently to move the lymphatic system and soothe irritated tissues. But when it comes to cataracts, we must understand the anatomy. A cataract forms inside the lens, not on the surface of the eye. This means that while castor oil may hydrate the eyelids, calm irritation, and support the delicate tissues around the eye, it cannot penetrate into the lens to dissolve a cataract. Some people do use sterile, ophthalmic castor oil for dry eyes, and it may help with moisture and tear-film stability. But it has not been shown in human studies to reverse cataracts. So the remedy remains valuable — just in the right place. Castor oil can support healing, but we must always match the treatment to the tissue involved. Use it wisely, use it safely, and always protect the precious gift of sight.

Barbara Oneill

130,462 Aufrufe • vor 9 Monaten

Are you tired of dealing with persistent eye problems that disrupt your day? Many of us ignore common issues like redness, itching, or blurriness, but these are often signs from your eyes that they need professional attention. Common Eye Symptoms You Shouldn't Ignore: - Red, Sore, or Itchy Eyes: Constant irritation that affects your comfort and concentration. - Light Sensitivity: Discomfort in bright light that causes you to squint and strain. - Glare and Halos at Night: A common sign of astigmatism that can make night driving difficult. - Excessive Tearing: Watery eyes that disrupt your day for no clear reason. - Digital Eye Strain: Vision blurring after using a computer or phone for extended periods. The Good News? These Conditions Are Treatable. At Kampala Eye & Lasik Hospital, we focus on the root cause of your discomfort, not just the symptoms. Our state-of-the-art facility is equipped with advanced diagnostic tools to provide an accurate diagnosis and a personalized treatment plan. Don't just live with daily eye problems. Get the expert care you deserve. Book your comprehensive eye examination today. +256 754 680980 | +256 775 642848 Acacia Mall, 3rd Floor

Are you tired of dealing with persistent eye problems that disrupt your day? Many of us ignore common issues like redness, itching, or blurriness, but these are often signs from your eyes that they need professional attention. Common Eye Symptoms You Shouldn't Ignore: - Red, Sore, or Itchy Eyes: Constant irritation that affects your comfort and concentration. - Light Sensitivity: Discomfort in bright light that causes you to squint and strain. - Glare and Halos at Night: A common sign of astigmatism that can make night driving difficult. - Excessive Tearing: Watery eyes that disrupt your day for no clear reason. - Digital Eye Strain: Vision blurring after using a computer or phone for extended periods. The Good News? These Conditions Are Treatable. At Kampala Eye & Lasik Hospital, we focus on the root cause of your discomfort, not just the symptoms. Our state-of-the-art facility is equipped with advanced diagnostic tools to provide an accurate diagnosis and a personalized treatment plan. Don't just live with daily eye problems. Get the expert care you deserve. Book your comprehensive eye examination today. +256 754 680980 | +256 775 642848 Acacia Mall, 3rd Floor

Kampala Eye and Lasik Hospital

314,423 Aufrufe • vor 11 Monaten

⚡️📣👇Tremendously excited to share our new Cell article, where we develop TriPath, a method for analyzing 3D pathology samples using weakly supervised AI. Article: TriPath enables 3D computational pathology via 3D multiple instance learning allowing AI models to capture intricate morphological details from pathology volumes. Code: Blog post: Tested on two different imaging modalities, and patient cohorts from two institutions. Our superstar Andrew H. Song put in a monumental effort of leading the study, in a fantastic collaboration with Jonathan Liu at University of Washington . Interesting aspects: - Utilizing the whole tissue volume and leveraging 3D deep learning enable superior risk prediction performance compared to 2D deep learning baselines based on a few sampled tissue sections that emulate standard clinical practice. This indicates TriPath can harness additional information provided by 3D tissue morphology. - The performance is also superior to clinical baselines from a reader study that involved six expert pathologists. - The morphologically heterogeneous tissue volume could lead to opposing patient-level outcome predictions, dependent on which portion of the tissue volume is used. This concurs with current clinical literature warning that tissue sampling bias can lead to misdiagnosis. Some limitations: - While the 3D pathology cohort size is unprecedented, it is smaller than typical 2D pathology cohorts. Further large-scale studies will be required for validation. Nevertheless, we believe that this study will initiate a positive cycle, encouraging academic institutions and pharmaceutical companies to contribute large banks of human tissue blocks with paired clinical outcomes, thus speeding up advancements in 3D computational pathology. Concluding insights: We believe that 3D pathology is just around the corner - It has the huge potential to not only augment/improve the current clinical practice centered around 2D examination of human tissue, but also help reveal novel biomarkers for prognosis and therapeutic response.. Harvard Medical School Harvard Data Science Initiative Mass General Brigham Broad Institute

⚡️📣👇Tremendously excited to share our new Cell article, where we develop TriPath, a method for analyzing 3D pathology samples using weakly supervised AI. Article: TriPath enables 3D computational pathology via 3D multiple instance learning allowing AI models to capture intricate morphological details from pathology volumes. Code: Blog post: Tested on two different imaging modalities, and patient cohorts from two institutions. Our superstar Andrew H. Song put in a monumental effort of leading the study, in a fantastic collaboration with Jonathan Liu at University of Washington . Interesting aspects: - Utilizing the whole tissue volume and leveraging 3D deep learning enable superior risk prediction performance compared to 2D deep learning baselines based on a few sampled tissue sections that emulate standard clinical practice. This indicates TriPath can harness additional information provided by 3D tissue morphology. - The performance is also superior to clinical baselines from a reader study that involved six expert pathologists. - The morphologically heterogeneous tissue volume could lead to opposing patient-level outcome predictions, dependent on which portion of the tissue volume is used. This concurs with current clinical literature warning that tissue sampling bias can lead to misdiagnosis. Some limitations: - While the 3D pathology cohort size is unprecedented, it is smaller than typical 2D pathology cohorts. Further large-scale studies will be required for validation. Nevertheless, we believe that this study will initiate a positive cycle, encouraging academic institutions and pharmaceutical companies to contribute large banks of human tissue blocks with paired clinical outcomes, thus speeding up advancements in 3D computational pathology. Concluding insights: We believe that 3D pathology is just around the corner - It has the huge potential to not only augment/improve the current clinical practice centered around 2D examination of human tissue, but also help reveal novel biomarkers for prognosis and therapeutic response.. Harvard Medical School Harvard Data Science Initiative Mass General Brigham Broad Institute

Faisal Mahmood

65,541 Aufrufe • vor 2 Jahren

The hallucinations of AI provide mental health break A new kind of AI is here. A real-time world model. This isn’t like anything else you’ve ever done on your computer. It creates frames one after another, which translates into a new kind of video generation engine. I’m using PixVerse’s R1, PixVerse which is a category-creating technology and has just been upgraded to 720P HD video. R1 can create anything you want. I asked it to take me skiing in the Swiss Alps. Unlike other engines like Kling or Seedance it creates interactive videos. What makes this real time world model different than others like Kling or Seedance? It builds video frames one after another, so you can actually interact with the video while it plays (and it doesn’t take one to three minutes to generate like the others). More about what I learned by talking with Pixverse in this thread →>

The hallucinations of AI provide mental health break A new kind of AI is here. A real-time world model. This isn’t like anything else you’ve ever done on your computer. It creates frames one after another, which translates into a new kind of video generation engine. I’m using PixVerse’s R1, PixVerse which is a category-creating technology and has just been upgraded to 720P HD video. R1 can create anything you want. I asked it to take me skiing in the Swiss Alps. Unlike other engines like Kling or Seedance it creates interactive videos. What makes this real time world model different than others like Kling or Seedance? It builds video frames one after another, so you can actually interact with the video while it plays (and it doesn’t take one to three minutes to generate like the others). More about what I learned by talking with Pixverse in this thread →>

Robert Scoble

12,932 Aufrufe • vor 5 Monaten